TY - JOUR
T1 - Insertion of a long KpnI family member within a mitochondrial-DNA-like sequence present in the human nuclear genome
AU - Shoji, Wakasugi
AU - Hisayuki, Nomiyama
AU - Makoto, Fukuda
AU - Teruhisa, Tsuzuki
AU - Kazunori, Shimada
N1 - Funding Information:
This work was supported by grants from the Ministry of Education, Science and Culture, Japan.
PY - 1985
Y1 - 1985
N2 - Structural analysis of a phage λ, Charon 4A clone carrying one of the human nuclear mitochondrial(mt)-DNA-like sequences revealed that a KpnI-family member (KpnI 5.5-kb DNA) is inserted within this sequence. The inserted KpnI 5.5-kb DNA contains several possible polyadenylation signal sequences followed by an A-rich sequence at its 3' end and is flanked by perfect 13-bp direct repeats of the duplicated mtDNA-like sequences. These structures strongly suggest that the KpnI 5.5-kb DNA is a mobile element. Comparison of the 5' terminal sequences of the KpnI 5.5-kb DNA and four other long KpnI-family DNAs so far examined, using the predicted general promoter sequence for eukaryotic tRNAs, indicates that they contain the consensus sequences for the split internal RNA polymerase III control region.
AB - Structural analysis of a phage λ, Charon 4A clone carrying one of the human nuclear mitochondrial(mt)-DNA-like sequences revealed that a KpnI-family member (KpnI 5.5-kb DNA) is inserted within this sequence. The inserted KpnI 5.5-kb DNA contains several possible polyadenylation signal sequences followed by an A-rich sequence at its 3' end and is flanked by perfect 13-bp direct repeats of the duplicated mtDNA-like sequences. These structures strongly suggest that the KpnI 5.5-kb DNA is a mobile element. Comparison of the 5' terminal sequences of the KpnI 5.5-kb DNA and four other long KpnI-family DNAs so far examined, using the predicted general promoter sequence for eukaryotic tRNAs, indicates that they contain the consensus sequences for the split internal RNA polymerase III control region.
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U2 - 10.1016/0378-1119(85)90183-0
DO - 10.1016/0378-1119(85)90183-0
M3 - Article
C2 - 3000880
AN - SCOPUS:0022199815
VL - 36
SP - 281
EP - 288
JO - Gene
JF - Gene
SN - 0378-1119
IS - 3
ER -