Insertion of a long KpnI family member within a mitochondrial-DNA-like sequence present in the human nuclear genome

Wakasugi Shoji; Nomiyama Hisayuki; Fukuda Makoto; Tsuzuki Teruhisa; Shimada Kazunori

doi:10.1016/0378-1119(85)90183-0

Insertion of a long KpnI family member within a mitochondrial-DNA-like sequence present in the human nuclear genome

Wakasugi Shoji, Nomiyama Hisayuki, Fukuda Makoto, Tsuzuki Teruhisa, Shimada Kazunori

Bioregulation

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Structural analysis of a phage λ, Charon 4A clone carrying one of the human nuclear mitochondrial(mt)-DNA-like sequences revealed that a KpnI-family member (KpnI 5.5-kb DNA) is inserted within this sequence. The inserted KpnI 5.5-kb DNA contains several possible polyadenylation signal sequences followed by an A-rich sequence at its 3' end and is flanked by perfect 13-bp direct repeats of the duplicated mtDNA-like sequences. These structures strongly suggest that the KpnI 5.5-kb DNA is a mobile element. Comparison of the 5' terminal sequences of the KpnI 5.5-kb DNA and four other long KpnI-family DNAs so far examined, using the predicted general promoter sequence for eukaryotic tRNAs, indicates that they contain the consensus sequences for the split internal RNA polymerase III control region.

Original language	English
Pages (from-to)	281-288
Number of pages	8
Journal	Gene
Volume	36
Issue number	3
DOIs	https://doi.org/10.1016/0378-1119(85)90183-0
Publication status	Published - 1985

All Science Journal Classification (ASJC) codes

Genetics

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title = "Insertion of a long KpnI family member within a mitochondrial-DNA-like sequence present in the human nuclear genome",

abstract = "Structural analysis of a phage λ, Charon 4A clone carrying one of the human nuclear mitochondrial(mt)-DNA-like sequences revealed that a KpnI-family member (KpnI 5.5-kb DNA) is inserted within this sequence. The inserted KpnI 5.5-kb DNA contains several possible polyadenylation signal sequences followed by an A-rich sequence at its 3' end and is flanked by perfect 13-bp direct repeats of the duplicated mtDNA-like sequences. These structures strongly suggest that the KpnI 5.5-kb DNA is a mobile element. Comparison of the 5' terminal sequences of the KpnI 5.5-kb DNA and four other long KpnI-family DNAs so far examined, using the predicted general promoter sequence for eukaryotic tRNAs, indicates that they contain the consensus sequences for the split internal RNA polymerase III control region.",

author = "Wakasugi Shoji and Nomiyama Hisayuki and Fukuda Makoto and Tsuzuki Teruhisa and Shimada Kazunori",

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T1 - Insertion of a long KpnI family member within a mitochondrial-DNA-like sequence present in the human nuclear genome

AU - Shoji, Wakasugi

AU - Hisayuki, Nomiyama

AU - Makoto, Fukuda

AU - Teruhisa, Tsuzuki

AU - Kazunori, Shimada

PY - 1985

Y1 - 1985

N2 - Structural analysis of a phage λ, Charon 4A clone carrying one of the human nuclear mitochondrial(mt)-DNA-like sequences revealed that a KpnI-family member (KpnI 5.5-kb DNA) is inserted within this sequence. The inserted KpnI 5.5-kb DNA contains several possible polyadenylation signal sequences followed by an A-rich sequence at its 3' end and is flanked by perfect 13-bp direct repeats of the duplicated mtDNA-like sequences. These structures strongly suggest that the KpnI 5.5-kb DNA is a mobile element. Comparison of the 5' terminal sequences of the KpnI 5.5-kb DNA and four other long KpnI-family DNAs so far examined, using the predicted general promoter sequence for eukaryotic tRNAs, indicates that they contain the consensus sequences for the split internal RNA polymerase III control region.

AB - Structural analysis of a phage λ, Charon 4A clone carrying one of the human nuclear mitochondrial(mt)-DNA-like sequences revealed that a KpnI-family member (KpnI 5.5-kb DNA) is inserted within this sequence. The inserted KpnI 5.5-kb DNA contains several possible polyadenylation signal sequences followed by an A-rich sequence at its 3' end and is flanked by perfect 13-bp direct repeats of the duplicated mtDNA-like sequences. These structures strongly suggest that the KpnI 5.5-kb DNA is a mobile element. Comparison of the 5' terminal sequences of the KpnI 5.5-kb DNA and four other long KpnI-family DNAs so far examined, using the predicted general promoter sequence for eukaryotic tRNAs, indicates that they contain the consensus sequences for the split internal RNA polymerase III control region.

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