Insertion sequence-excision enhancer removes transposable elements from bacterial genomes and induces various genomic deletions

Masahiro Kusumoto, Tadasuke Ooka, Yoshiaki Nishiya, Yoshitoshi Ogura, Takashi Saito, Yasuhiko Sekine, Taketoshi Iwata, Masato Akiba, Tetsuya Hayashi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Insertion sequences (ISs) are the simplest transposable elements and are widely distributed in bacteria. It has long been thought that IS excision rarely occurs in bacterial cells because most bacteria exhibit no end-joining activity to regenerate donor DNA after IS excision. Recently, however, we found that excision of IS629, an IS3 family member, occurs frequently in Escherichia coli O157. In this paper, we describe a protein IS-excision enhancer (IEE) that promotes IS629 excision from the O157 genome in an IS transposase-dependent manner. Various types of genomic deletions are also generated on IEE-mediated IS excision, and IEE promotes the excision of other IS3 family members and ISs from several other IS families. These data and the phylogeny of IEE homologues found in a broad range of bacteria suggest that IEE proteins have coevolved with IS elements and have pivotal roles in bacterial genome evolution by inducing IS removal and genomic deletion.

Original languageEnglish
Article number152
JournalNature communications
Volume2
Issue number1
DOIs
Publication statusPublished - Jan 18 2011
Externally publishedYes

Fingerprint

Bacterial Genomes
deletion
DNA Transposable Elements
genome
Insertional Mutagenesis
insertion
Bacteria
Genes
Transposases
Joining
Escherichia coli
bacteria
Proteins
DNA
proteins
Escherichia coli O157
Phylogeny
Escherichia
Genome
deoxyribonucleic acid

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Insertion sequence-excision enhancer removes transposable elements from bacterial genomes and induces various genomic deletions. / Kusumoto, Masahiro; Ooka, Tadasuke; Nishiya, Yoshiaki; Ogura, Yoshitoshi; Saito, Takashi; Sekine, Yasuhiko; Iwata, Taketoshi; Akiba, Masato; Hayashi, Tetsuya.

In: Nature communications, Vol. 2, No. 1, 152, 18.01.2011.

Research output: Contribution to journalArticle

Kusumoto, Masahiro ; Ooka, Tadasuke ; Nishiya, Yoshiaki ; Ogura, Yoshitoshi ; Saito, Takashi ; Sekine, Yasuhiko ; Iwata, Taketoshi ; Akiba, Masato ; Hayashi, Tetsuya. / Insertion sequence-excision enhancer removes transposable elements from bacterial genomes and induces various genomic deletions. In: Nature communications. 2011 ; Vol. 2, No. 1.
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abstract = "Insertion sequences (ISs) are the simplest transposable elements and are widely distributed in bacteria. It has long been thought that IS excision rarely occurs in bacterial cells because most bacteria exhibit no end-joining activity to regenerate donor DNA after IS excision. Recently, however, we found that excision of IS629, an IS3 family member, occurs frequently in Escherichia coli O157. In this paper, we describe a protein IS-excision enhancer (IEE) that promotes IS629 excision from the O157 genome in an IS transposase-dependent manner. Various types of genomic deletions are also generated on IEE-mediated IS excision, and IEE promotes the excision of other IS3 family members and ISs from several other IS families. These data and the phylogeny of IEE homologues found in a broad range of bacteria suggest that IEE proteins have coevolved with IS elements and have pivotal roles in bacterial genome evolution by inducing IS removal and genomic deletion.",
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