Insertion/deletion polymorphism in clusterin gene influences serum lipid levels and carotid intima-media thickness in hypertensive Japanese females

Yoshikazu Miwa, Shin Takiuchi, Kei Kamide, Masayoshi Yoshii, Takeshi Horio, Chihiro Tanaka, Mariko Banno, Toshiyuki Miyata, Toshiyuki Sasaguri, Yuhei Kawano

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Clusterin has been implicated in lipid metabolism and atherogenesis, however, the influence of genetic variation has not been examined in Japanese. In this study, we identified 11 single nucleotide polymorphisms (SNPs) of clusterin gene by direct sequencing. Among them, one promoter SNP (-4453T > G), one missense SNP (4183G > A), and 2 common SNPs (5608T > C and 6316delT) were genotyped in 525 asymptomatic hypertensives not treated with lipid lowering agents. -4453T > G, 4183G > A, and 5608T > C showed no correlation with the clinical characteristics, however, in the 6316delT, an insertion (I)/deletion (D) polymorphism, D/D subjects had significantly higher levels of total cholesterol and low-density lipoprotein (LDL)-cholesterol than I/I subjects in females but not in males. Female subjects with the D allele (D/D + I/D) had greater intima-media thickness of the carotid artery than I/I subjects. In a multiple logistic regression analysis, the D allele of 6316delT was detected as an independent predictor for the plaque prevalence. In conclusion, the clusterin gene polymorphism may contribute to the serum lipid levels and the progression of carotid atherosclerosis in hypertensive Japanese females.

Original languageEnglish
Pages (from-to)1587-1593
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume331
Issue number4
DOIs
Publication statusPublished - Jun 17 2005

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this