TY - JOUR
T1 - Insight into functional diversity of cytochrome P450 in the white-rot basidiomycete Phanerochaete chrysosporium
T2 - Involvement of versatile monooxygenase
AU - Hirosue, Shinji
AU - Tazaki, Masahiro
AU - Hiratsuka, Nobuhiro
AU - Yanai, Satoshi
AU - Kabumoto, Hiroki
AU - Shinkyo, Raku
AU - Arisawa, Akira
AU - Sakaki, Toshiyuki
AU - Tsunekawa, Hiroshi
AU - Johdo, Osamu
AU - Ichinose, Hirofumi
AU - Wariishi, Hiroyuki
N1 - Funding Information:
We are deeply grateful to Professor Osamu Gotoh (Kyoto University, Japan) for supporting bioinformatic gene prediction of PcCYPs. This research was supported in part by a Grant-in-Aid (No. 21688013 ) for Young Scientists (A) from the Ministry of Education, Culture, Sports, Science and Technology, Japan (to H.I.).
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/4/1
Y1 - 2011/4/1
N2 - To elucidate functional diversity of cytochrome P450 monooxygenases from the white-rot basidiomycete Phanerochaete chrysosporium (PcCYPs), we conducted a comprehensive functional screening using a wide variety of compounds. A functionomic survey resulted in characterization of novel PcCYP functions and discovery of versatile PcCYPs that exhibit broad substrate profiles. These results suggested that multifunctional properties of the versatile PcCYPs would play crucial roles in diversification of fungal metabolic systems involved in xenobiotic detoxification. To our knowledge, this is the first report describing multifunctional properties of versatile P450s from the fungal kingdom. An increased compilation of PcCYP functions will facilitate a thorough understanding of metabolic diversity in basidiomycetes and provide new insights that could also expedite practical applications in the biotechnology sector.
AB - To elucidate functional diversity of cytochrome P450 monooxygenases from the white-rot basidiomycete Phanerochaete chrysosporium (PcCYPs), we conducted a comprehensive functional screening using a wide variety of compounds. A functionomic survey resulted in characterization of novel PcCYP functions and discovery of versatile PcCYPs that exhibit broad substrate profiles. These results suggested that multifunctional properties of the versatile PcCYPs would play crucial roles in diversification of fungal metabolic systems involved in xenobiotic detoxification. To our knowledge, this is the first report describing multifunctional properties of versatile P450s from the fungal kingdom. An increased compilation of PcCYP functions will facilitate a thorough understanding of metabolic diversity in basidiomycetes and provide new insights that could also expedite practical applications in the biotechnology sector.
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U2 - 10.1016/j.bbrc.2011.02.121
DO - 10.1016/j.bbrc.2011.02.121
M3 - Article
C2 - 21362401
AN - SCOPUS:79953163565
VL - 407
SP - 118
EP - 123
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -