Insight into functional diversity of cytochrome P450 in the white-rot basidiomycete Phanerochaete chrysosporium: Involvement of versatile monooxygenase

Shinji Hirosue, Masahiro Tazaki, Nobuhiro Hiratsuka, Satoshi Yanai, Hiroki Kabumoto, Raku Shinkyo, Akira Arisawa, Toshiyuki Sakaki, Hiroshi Tsunekawa, Osamu Johdo, Hirofumi Ichinose, Hiroyuki Wariishi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

To elucidate functional diversity of cytochrome P450 monooxygenases from the white-rot basidiomycete Phanerochaete chrysosporium (PcCYPs), we conducted a comprehensive functional screening using a wide variety of compounds. A functionomic survey resulted in characterization of novel PcCYP functions and discovery of versatile PcCYPs that exhibit broad substrate profiles. These results suggested that multifunctional properties of the versatile PcCYPs would play crucial roles in diversification of fungal metabolic systems involved in xenobiotic detoxification. To our knowledge, this is the first report describing multifunctional properties of versatile P450s from the fungal kingdom. An increased compilation of PcCYP functions will facilitate a thorough understanding of metabolic diversity in basidiomycetes and provide new insights that could also expedite practical applications in the biotechnology sector.

Original languageEnglish
Pages (from-to)118-123
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume407
Issue number1
DOIs
Publication statusPublished - Apr 1 2011

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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