Insights into the micromechanical properties of the metaphase spindle

Yuta Shimamoto, Yusuke T. Maeda, Shin'Ichi Ishiwata, Albert J. Libchaber, Tarun M. Kapoor

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The microtubule-based metaphase spindle is subjected to forces that act in diverse orientations and over a wide range of timescales. Currently, we cannot explain how this dynamic structure generates and responds to forces while maintaining overall stability, as we have a poor understanding of its micromechanical properties. Here, we combine the use of force-calibrated needles, high-resolution microscopy, and biochemical perturbations to analyze the vertebrate metaphase spindle's timescale- and orientation-dependent viscoelastic properties. We find that spindle viscosity depends on microtubule crosslinking and density. Spindle elasticity can be linked to kinetochore and nonkinetochore microtubule rigidity, and also to spindle pole organization by kinesin-5 and dynein. These data suggest a quantitative model for the micromechanics of this cytoskeletal architecture and provide insight into how structural and functional stability is maintained in the face of forces, such as those that control spindle size and position, and can result from deformations associated with chromosome movement. PaperFlick:

Original languageEnglish
Pages (from-to)1062-1074
Number of pages13
JournalCell
Volume145
Issue number7
DOIs
Publication statusPublished - Jun 24 2011
Externally publishedYes

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Metaphase
Microtubules
Dyneins
Kinesin
Micromechanics
Chromosomes
Rigidity
Needles
Crosslinking
Spindle Poles
Kinetochores
Elasticity
Poles
Microscopic examination
Viscosity
Vertebrates
Microscopy

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Shimamoto, Y., Maeda, Y. T., Ishiwata, SI., Libchaber, A. J., & Kapoor, T. M. (2011). Insights into the micromechanical properties of the metaphase spindle. Cell, 145(7), 1062-1074. https://doi.org/10.1016/j.cell.2011.05.038

Insights into the micromechanical properties of the metaphase spindle. / Shimamoto, Yuta; Maeda, Yusuke T.; Ishiwata, Shin'Ichi; Libchaber, Albert J.; Kapoor, Tarun M.

In: Cell, Vol. 145, No. 7, 24.06.2011, p. 1062-1074.

Research output: Contribution to journalArticle

Shimamoto, Y, Maeda, YT, Ishiwata, SI, Libchaber, AJ & Kapoor, TM 2011, 'Insights into the micromechanical properties of the metaphase spindle', Cell, vol. 145, no. 7, pp. 1062-1074. https://doi.org/10.1016/j.cell.2011.05.038
Shimamoto Y, Maeda YT, Ishiwata SI, Libchaber AJ, Kapoor TM. Insights into the micromechanical properties of the metaphase spindle. Cell. 2011 Jun 24;145(7):1062-1074. https://doi.org/10.1016/j.cell.2011.05.038
Shimamoto, Yuta ; Maeda, Yusuke T. ; Ishiwata, Shin'Ichi ; Libchaber, Albert J. ; Kapoor, Tarun M. / Insights into the micromechanical properties of the metaphase spindle. In: Cell. 2011 ; Vol. 145, No. 7. pp. 1062-1074.
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