Abstract
Background and Objectives: Chromosomal instability is a common feature of pancreatic carcinoma, but its biological significance remains unclear. In this study, we investigated the association between chromosomal instability and biological aggressiveness in human pancreatic cancer cells. Methods: Fluorescence in situ hybridization was performed to examine changes in chromosomal numbers in a total of 13 pancreatic cancer cell lines. We also assessed the potential for tumor aggressiveness within cancer cells by in vitro migration and invasion assay and by subcutaneous implantation into nude mice. Results: Chromosomal instability, characterized by numerical variations in copy numbers of chromosome 8, was observed in most cell lines, and the magnitude of instability was correlated well with both motility (P < 0.001) and invasion rate (P < 0.001) of these cells. Furthermore, a significant positive correlation existed between chromosome instability and tumor growth in vivo (P < 0.01). Conclusions: These results suggest that the increased level of chromosomal instability may play a critical role in the development of aggressive tumor phenotype during pancreatic cancer progression.
| Original language | English |
|---|---|
| Pages (from-to) | 181-187 |
| Number of pages | 7 |
| Journal | Journal of Surgical Oncology |
| Volume | 76 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Apr 5 2001 |
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All Science Journal Classification (ASJC) codes
- Surgery
- Oncology
Cite this
Instability of chromosome 8 as an indicator of aggressive tumor phenotype in pancreatic cancer. / Sato, Norihiro; Mizumoto, Kazuhiro; Maehara, Naoki; Shono, Masaki; Nagai, Eishi; Tanaka, Masao.
In: Journal of Surgical Oncology, Vol. 76, No. 3, 05.04.2001, p. 181-187.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Instability of chromosome 8 as an indicator of aggressive tumor phenotype in pancreatic cancer
AU - Sato, Norihiro
AU - Mizumoto, Kazuhiro
AU - Maehara, Naoki
AU - Shono, Masaki
AU - Nagai, Eishi
AU - Tanaka, Masao
PY - 2001/4/5
Y1 - 2001/4/5
N2 - Background and Objectives: Chromosomal instability is a common feature of pancreatic carcinoma, but its biological significance remains unclear. In this study, we investigated the association between chromosomal instability and biological aggressiveness in human pancreatic cancer cells. Methods: Fluorescence in situ hybridization was performed to examine changes in chromosomal numbers in a total of 13 pancreatic cancer cell lines. We also assessed the potential for tumor aggressiveness within cancer cells by in vitro migration and invasion assay and by subcutaneous implantation into nude mice. Results: Chromosomal instability, characterized by numerical variations in copy numbers of chromosome 8, was observed in most cell lines, and the magnitude of instability was correlated well with both motility (P < 0.001) and invasion rate (P < 0.001) of these cells. Furthermore, a significant positive correlation existed between chromosome instability and tumor growth in vivo (P < 0.01). Conclusions: These results suggest that the increased level of chromosomal instability may play a critical role in the development of aggressive tumor phenotype during pancreatic cancer progression.
AB - Background and Objectives: Chromosomal instability is a common feature of pancreatic carcinoma, but its biological significance remains unclear. In this study, we investigated the association between chromosomal instability and biological aggressiveness in human pancreatic cancer cells. Methods: Fluorescence in situ hybridization was performed to examine changes in chromosomal numbers in a total of 13 pancreatic cancer cell lines. We also assessed the potential for tumor aggressiveness within cancer cells by in vitro migration and invasion assay and by subcutaneous implantation into nude mice. Results: Chromosomal instability, characterized by numerical variations in copy numbers of chromosome 8, was observed in most cell lines, and the magnitude of instability was correlated well with both motility (P < 0.001) and invasion rate (P < 0.001) of these cells. Furthermore, a significant positive correlation existed between chromosome instability and tumor growth in vivo (P < 0.01). Conclusions: These results suggest that the increased level of chromosomal instability may play a critical role in the development of aggressive tumor phenotype during pancreatic cancer progression.
UR - http://www.scopus.com/inward/record.url?scp=0035078232&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035078232&partnerID=8YFLogxK
U2 - 10.1002/jso.1032
DO - 10.1002/jso.1032
M3 - Article
C2 - 11276022
AN - SCOPUS:0035078232
VL - 76
SP - 181
EP - 187
JO - Journal of Surgical Oncology
JF - Journal of Surgical Oncology
SN - 0022-4790
IS - 3
ER -