Intact glycation end products containing carboxymethyl-lysine and glyoxal lysine dimer obtained from synthetic collagen model peptide

Hiroaki Yamada, Tomoko Sasaki, Sachiko Niwa, Tohru Oishi, Michio Murata, Toru Kawakami, Saburo Aimoto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Glycation reactions using a model peptide of collagen and glucose or ribose resulted in detection of carboxylmethyl-lysine in the peptide; and treatment with glyoxal provided a dimer of the peptide linked with glyoxal lysine dimer (GOLD). Advanced glycation end products (AGE) are accumulated in human tissues when long-lived proteins are glycated due to hyperglycemia and/or aging. In this study, we synthesized a collagen model peptide, Ac-(Pro-Hyp-Gly) 5-Pro-Lys-Gly-(Pro-Hyp-Gly) 5-Ala-NH 2 to investigate intact AGEs in peptides. The peptide formed a stable triple helix structure, and was subjected to glycation reactions with glucose, ribose and glyoxal. Besides carboxymethyl-lysine in the peptide, a conjugated form linked with glyoxal lysine dimer (GOLD) was detected upon treatment with glyoxal. This is the first example of intact glycation-derived dimers of peptides retaining intrinsic protein structures.

Original languageEnglish
Pages (from-to)5677-5680
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume14
Issue number22
DOIs
Publication statusPublished - Nov 15 2004
Externally publishedYes

Fingerprint

Glyoxal
Dimers
Lysine
Collagen
Peptides
Ribose
Glucose
Advanced Glycosylation End Products
N(6)-carboxymethyllysine
Hyperglycemia
Proteins
Aging of materials
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Intact glycation end products containing carboxymethyl-lysine and glyoxal lysine dimer obtained from synthetic collagen model peptide. / Yamada, Hiroaki; Sasaki, Tomoko; Niwa, Sachiko; Oishi, Tohru; Murata, Michio; Kawakami, Toru; Aimoto, Saburo.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 14, No. 22, 15.11.2004, p. 5677-5680.

Research output: Contribution to journalArticle

Yamada, Hiroaki ; Sasaki, Tomoko ; Niwa, Sachiko ; Oishi, Tohru ; Murata, Michio ; Kawakami, Toru ; Aimoto, Saburo. / Intact glycation end products containing carboxymethyl-lysine and glyoxal lysine dimer obtained from synthetic collagen model peptide. In: Bioorganic and Medicinal Chemistry Letters. 2004 ; Vol. 14, No. 22. pp. 5677-5680.
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AU - Niwa, Sachiko

AU - Oishi, Tohru

AU - Murata, Michio

AU - Kawakami, Toru

AU - Aimoto, Saburo

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