Integrated MALDI-MS imaging and LC-MS techniques for visualizing spatiotemporal metabolomic dynamics in a rat stroke model

Miho Irie, Yoshinori Fujimura, Mayumi Yamato, Daisuke Miura, Hiroyuki Wariishi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Spatiotemporal information about biomolecules is indispensable for precise pathological analysis, but it remains largely unclear. Here we show a novel analytical platform combing mass spectrometry imaging (MSI) with its complementary technique, liquid chromatography-mass spectrometry (LC-MS), to elucidate more comprehensive metabolic behaviors, with spatiotemporal information, in tissues. Analysis of a rat transient middle cerebral artery occlusion (MCAO) brain tissue after ischemia-reperfusion was performed to characterize the detailed metabolomic response to pathological alterations. To compare the spatially resolved metabolic state between ischemic and contralateral hemispheres of the MCAO brain, coronally sliced tissues were subjected to MSI. We also measured the metabolites extracted from three different cerebral regions, including whole cortex (CTX), hippocampus (HI) and corpus striatum (CPu), by LC-MS. In the ischemic hemisphere, significant metabolic changes at the CTX and CPu were observed after reperfusion, while not at the HI. A region-specific metabolic behavior was observed in amino acid and nucleotide metabolism, as well as in the TCA cycle. Correlation between MSI and LC-MS data was relatively high in the CTX and CPu. Combination of both MS platforms visualized the diverse spatiotemporal metabolic dynamics during pathological progress. Thus, our proposed strategy will contribute to the understanding of the complex pathogenesis of ischemia-reperfusion.

Original languageEnglish
Pages (from-to)473-483
Number of pages11
JournalMetabolomics
Volume10
Issue number3
DOIs
Publication statusPublished - Jan 1 2014

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Metabolomics
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Liquid chromatography
Liquid Chromatography
Mass spectrometry
Rats
Mass Spectrometry
Stroke
Imaging techniques
Corpus Striatum
Reperfusion
Middle Cerebral Artery Infarction
Tissue
Hippocampus
Brain
Ischemia
Biomolecules
Metabolites
Metabolism
Nucleotides

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry

Cite this

Integrated MALDI-MS imaging and LC-MS techniques for visualizing spatiotemporal metabolomic dynamics in a rat stroke model. / Irie, Miho; Fujimura, Yoshinori; Yamato, Mayumi; Miura, Daisuke; Wariishi, Hiroyuki.

In: Metabolomics, Vol. 10, No. 3, 01.01.2014, p. 473-483.

Research output: Contribution to journalArticle

Irie, Miho ; Fujimura, Yoshinori ; Yamato, Mayumi ; Miura, Daisuke ; Wariishi, Hiroyuki. / Integrated MALDI-MS imaging and LC-MS techniques for visualizing spatiotemporal metabolomic dynamics in a rat stroke model. In: Metabolomics. 2014 ; Vol. 10, No. 3. pp. 473-483.
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