TY - JOUR
T1 - Integrated Omics Approaches Revealed the Osmotic Stress-Responsive Genes and Microbiota in Gill of Marine Medaka
AU - Lai, Keng Po
AU - Zhu, Peng
AU - Boncan, Delbert Almerick T.
AU - Yang, Lu
AU - Leung, Cherry Chi Tim
AU - Ho, Jeff Cheuk Hin
AU - Lin, Xiao
AU - Chan, Ting Fung
AU - Kong, Richard Yuen Chong
AU - Tse, William Ka Fai
N1 - Funding Information:
The osmoregulatory works in the W.K.F.T. group are partially supported by the Cooperative Program of Atmosphere and Ocean Research Institute, The University of Tokyo (123-2018, 127-2019; 2021), Japan, and the Individual Research Collaboration Project, National Institute of Basic Biology, Japan (17-314, 18-319). This work was supported in part by grants from the National Natural Science Foundation of China (no. 82160282 to K.P.L. and no. 41977371 to R.Y.C.K.).
Publisher Copyright:
Copyright © 2022 Lai et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license.
PY - 2022/4
Y1 - 2022/4
N2 - Aquatic fishes face osmotic stress continuously, and the gill is the first tissue that senses and responds to the external osmotic challenges. However, the understandings of how the gill microbiota could respond to osmotic stress and their potential host-bacterium relationships are limited. The objectives of the current study are to identify the hypotonic responsive genes in the gill cells and profile the gill microbiota communities after fresh water transfer experiment via transcriptome sequencing and 16S rRNA gene sequencing. Transcriptome sequencing identified 1,034 differentially expressed genes (DEGs), such as aquaporin and sodium potassium chloride cotransporter, after the fresh water transfer. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis further highlighted the steroid biosynthesis and glycosaminoglycan biosynthesis pathways in the gill. Moreover, the 16S rRNA gene sequencing identified Vibrio as the dominant bacterium in the seawater, which changed to Pseudomonas and Cetobacterium after the fresh water transfer. The alpha diversity analysis suggested that the gill bacterial diversity was lower in the fresh water transferred group. The KEGG and MetaCyc analysis further predicted the alteration of the glycosaminoglycan and chitin metabolisms in the gill bacteria. Collectively, the common glycosaminoglycan and chitin pathways in both the gill cells and gill microbiota suggest the host-bacterium interaction in gill facilitates the fresh water acclimation.
AB - Aquatic fishes face osmotic stress continuously, and the gill is the first tissue that senses and responds to the external osmotic challenges. However, the understandings of how the gill microbiota could respond to osmotic stress and their potential host-bacterium relationships are limited. The objectives of the current study are to identify the hypotonic responsive genes in the gill cells and profile the gill microbiota communities after fresh water transfer experiment via transcriptome sequencing and 16S rRNA gene sequencing. Transcriptome sequencing identified 1,034 differentially expressed genes (DEGs), such as aquaporin and sodium potassium chloride cotransporter, after the fresh water transfer. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis further highlighted the steroid biosynthesis and glycosaminoglycan biosynthesis pathways in the gill. Moreover, the 16S rRNA gene sequencing identified Vibrio as the dominant bacterium in the seawater, which changed to Pseudomonas and Cetobacterium after the fresh water transfer. The alpha diversity analysis suggested that the gill bacterial diversity was lower in the fresh water transferred group. The KEGG and MetaCyc analysis further predicted the alteration of the glycosaminoglycan and chitin metabolisms in the gill bacteria. Collectively, the common glycosaminoglycan and chitin pathways in both the gill cells and gill microbiota suggest the host-bacterium interaction in gill facilitates the fresh water acclimation.
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U2 - 10.1128/msystems.00047-22
DO - 10.1128/msystems.00047-22
M3 - Article
AN - SCOPUS:85129056356
SN - 2379-5077
VL - 7
JO - mSystems
JF - mSystems
IS - 2
ER -
