Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device

Yuji Nashimoto, Tomoya Hayashi, Itsuki Kunita, Akiko Nakamasu, Yu Suke Torisawa, Masamune Nakayama, Hisako Imamura, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Creating vascular networks in tissues is crucial for tissue engineering. Although recent studies have demonstrated the formation of vessel-like structures in a tissue model, long-term culture is still challenging due to the lack of active perfusion in vascular networks. Here, we present a method to create a three-dimensional cellular spheroid with a perfusable vascular network in a microfluidic device. By the definition of the cellular interaction between human lung fibroblasts (hLFs) in a spheroid and human umbilical vein endothelial cells (HUVECs) in microchannels, angiogenic sprouts were induced from microchannels toward the spheroid; the sprouts reached the vessel-like structures in a spheroid to form a continuous lumen. We demonstrated that the vascular network could administer biological substances to the interior of the spheroid. As cell density in the spheroid is similar to that of a tissue, the perfusable vasculature model opens up new possibilities for a long-term tissue culture in vitro.

Original languageEnglish
Pages (from-to)506-518
Number of pages13
JournalIntegrative Biology (United Kingdom)
Volume9
Issue number6
DOIs
Publication statusPublished - Jun 1 2017

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Lab-On-A-Chip Devices
Microfluidics
Blood Vessels
Tissue
Microchannels
Tissue culture
Endothelial cells
Cellular Spheroids
Fibroblasts
Tissue engineering
Human Umbilical Vein Endothelial Cells
Tissue Engineering
Perfusion
Cell Count
Lung

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

Cite this

Nashimoto, Y., Hayashi, T., Kunita, I., Nakamasu, A., Torisawa, Y. S., Nakayama, M., ... Yokokawa, R. (2017). Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device. Integrative Biology (United Kingdom), 9(6), 506-518. https://doi.org/10.1039/c7ib00024c

Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device. / Nashimoto, Yuji; Hayashi, Tomoya; Kunita, Itsuki; Nakamasu, Akiko; Torisawa, Yu Suke; Nakayama, Masamune; Imamura, Hisako; Kotera, Hidetoshi; Nishiyama, Koichi; Miura, Takashi; Yokokawa, Ryuji.

In: Integrative Biology (United Kingdom), Vol. 9, No. 6, 01.06.2017, p. 506-518.

Research output: Contribution to journalArticle

Nashimoto, Y, Hayashi, T, Kunita, I, Nakamasu, A, Torisawa, YS, Nakayama, M, Imamura, H, Kotera, H, Nishiyama, K, Miura, T & Yokokawa, R 2017, 'Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device', Integrative Biology (United Kingdom), vol. 9, no. 6, pp. 506-518. https://doi.org/10.1039/c7ib00024c
Nashimoto, Yuji ; Hayashi, Tomoya ; Kunita, Itsuki ; Nakamasu, Akiko ; Torisawa, Yu Suke ; Nakayama, Masamune ; Imamura, Hisako ; Kotera, Hidetoshi ; Nishiyama, Koichi ; Miura, Takashi ; Yokokawa, Ryuji. / Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device. In: Integrative Biology (United Kingdom). 2017 ; Vol. 9, No. 6. pp. 506-518.
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