Integration of transcriptome and methylome analysis of aldosterone-producing adenomas

Masanori Murakami, Takanobu Yoshimoto, Kazuhiko Nakabayashi, Kyoichiro Tsuchiya, Isao Minami, Ryotaro Bouchi, Hajime Izumiyama, Yasuhisa Fujii, Kosei Abe, Chiharu Tayama, Koshi Hashimoto, Takayoshi Suganami, Ken Ichiro Hata, Kazunori Kihara, Yoshihiro Ogawa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Objective: The pathophysiology of aldosterone-producing adenomas (APA) has been investigated intensively through genetic and genomic approaches. However, the role of epigenetics in APA is not fully understood. In the present study, we explored the relationship between gene expression and DNA methylation status in APA. Methods: We conducted an integrated analysis of transcriptome and methylome data of paired APA-adjacent adrenal gland (AAG) samples from the same patient. The adrenal specimens were obtained from seven Japanese patients with APA who underwent adrenalectomy. Gene expression and genome-wide CpG methylation profiles were obtained from RNA and DNA samples that were extracted from those seven paired tissues. Results: Methylome analysis showed global CpG hypomethylation in APA relative to AAG. The integration of gene expression and methylation status showed that 34 genes were up-regulated with CpG hypomethylation in APA. Of these, three genes (CYP11B2, MC2R, and HPX) may be related to aldosterone production, and five genes (PRRX1, RAB38, FAP, GCNT2, and ASB4) are potentially involved in tumorigenesis. Conclusion: The present study is the first methylome analysis to compare APA with AAG in the same patients. Our integrated analysis of transcriptome and methylome revealed DNA hypomethylation in APA and identified several up-regulated genes with DNA hypomethylation that may be involved in aldosterone production and tumorigenesis.

Original languageEnglish
Pages (from-to)185-195
Number of pages11
JournalEuropean Journal of Endocrinology
Volume173
Issue number2
DOIs
Publication statusPublished - Aug 1 2015

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Gene Expression Profiling
Aldosterone
Adenoma
Adrenal Glands
Gene Expression
Methylation
Genes
DNA
Carcinogenesis
Cytochrome P-450 CYP11B2
Adrenalectomy
DNA Methylation
Epigenomics
Genome
RNA

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Murakami, M., Yoshimoto, T., Nakabayashi, K., Tsuchiya, K., Minami, I., Bouchi, R., ... Ogawa, Y. (2015). Integration of transcriptome and methylome analysis of aldosterone-producing adenomas. European Journal of Endocrinology, 173(2), 185-195. https://doi.org/10.1530/EJE-15-0148

Integration of transcriptome and methylome analysis of aldosterone-producing adenomas. / Murakami, Masanori; Yoshimoto, Takanobu; Nakabayashi, Kazuhiko; Tsuchiya, Kyoichiro; Minami, Isao; Bouchi, Ryotaro; Izumiyama, Hajime; Fujii, Yasuhisa; Abe, Kosei; Tayama, Chiharu; Hashimoto, Koshi; Suganami, Takayoshi; Hata, Ken Ichiro; Kihara, Kazunori; Ogawa, Yoshihiro.

In: European Journal of Endocrinology, Vol. 173, No. 2, 01.08.2015, p. 185-195.

Research output: Contribution to journalArticle

Murakami, M, Yoshimoto, T, Nakabayashi, K, Tsuchiya, K, Minami, I, Bouchi, R, Izumiyama, H, Fujii, Y, Abe, K, Tayama, C, Hashimoto, K, Suganami, T, Hata, KI, Kihara, K & Ogawa, Y 2015, 'Integration of transcriptome and methylome analysis of aldosterone-producing adenomas', European Journal of Endocrinology, vol. 173, no. 2, pp. 185-195. https://doi.org/10.1530/EJE-15-0148
Murakami M, Yoshimoto T, Nakabayashi K, Tsuchiya K, Minami I, Bouchi R et al. Integration of transcriptome and methylome analysis of aldosterone-producing adenomas. European Journal of Endocrinology. 2015 Aug 1;173(2):185-195. https://doi.org/10.1530/EJE-15-0148
Murakami, Masanori ; Yoshimoto, Takanobu ; Nakabayashi, Kazuhiko ; Tsuchiya, Kyoichiro ; Minami, Isao ; Bouchi, Ryotaro ; Izumiyama, Hajime ; Fujii, Yasuhisa ; Abe, Kosei ; Tayama, Chiharu ; Hashimoto, Koshi ; Suganami, Takayoshi ; Hata, Ken Ichiro ; Kihara, Kazunori ; Ogawa, Yoshihiro. / Integration of transcriptome and methylome analysis of aldosterone-producing adenomas. In: European Journal of Endocrinology. 2015 ; Vol. 173, No. 2. pp. 185-195.
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AU - Minami, Isao

AU - Bouchi, Ryotaro

AU - Izumiyama, Hajime

AU - Fujii, Yasuhisa

AU - Abe, Kosei

AU - Tayama, Chiharu

AU - Hashimoto, Koshi

AU - Suganami, Takayoshi

AU - Hata, Ken Ichiro

AU - Kihara, Kazunori

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