Intensive nursing and feeding during the early growth period altered intramuscular adipogenesis in crossbred steers (Japanese Black male x Holstein female)

Fumio Ebara, Sunao Inada, So Hei Asaoka, Yoshihiro Isozaki, Akira Saito, Tetsuji Etoh, Yuji Shiotsuka, Takafumi Gotoh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We investigated the influence of feed quality during the early growth stage on expression of adipogenic genes in skeletal muscle of crossbred cattle. The cattle were divided three groups at 45 days of age group high energy (H: n = 7), group normal energy (N: n = 7) and group roughage (R: n = 7). Cattle in group H were three fold-intensively fed a high protein and low fat milk replacer (Crude Protein (CP): 28%, Ether Extracts (EE): 18%, max: 2.0 kg, 12 L day-1) from 45 days to 4 months of age. In groups N and R, cattle were fed a normal milk replacer (CP: 25%, EE: 23%, max: 0.5 kg, 4 L day-1) from 45 days to 4 months of age. From 4-10 months of age, cattle in group H were fed a high-nutrition Total Mixture Rate (TMR), while group N were fed a normal-nutrition TMR and group R were fed only roughage. Muscle samples were taken by biopsy from Longissimus thoracis muscle (LT) at 45 days, 4 and 10 months of age. Results from assays using quantitative real-time Polym erase Chain Reaction (PCR) revealed that gene expression of Peroxisome Proliferator-activated Receptor Gamma 2 (PPARγ2), CCAAT/Enhancer-Binding Protein alpha (C/EBPα), Stearoyl-CoA Desaturase (SCD), Glucose-6-Phosphate Dehydrogenase (G6PD), Fatty Acid Synthase (FASN) and Leptin related to adipogenesis and fatty acid synthesis differed among groups. The expression of these genes was significantly different among groups at 10 months but not at 4 months of age. These results demonstrated that differences in feed quality during early growth stages had substantial effects on the expression of genes related to adipogenesis and fatty acid synthesis in LT.

Original languageEnglish
Pages (from-to)982-989
Number of pages8
JournalJournal of Animal and Veterinary Advances
Volume9
Issue number6
DOIs
Publication statusPublished - Jul 21 2010

Fingerprint

Adipogenesis
Wagyu
breast feeding
crossbreds
Nursing
Holstein
Gene Expression
Growth
cattle
Dietary Fiber
feed quality
milk replacer
Muscles
Ether
Milk
longissimus muscle
Fatty Acids
gene expression
muscles
Stearoyl-CoA Desaturase

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • veterinary(all)

Cite this

Intensive nursing and feeding during the early growth period altered intramuscular adipogenesis in crossbred steers (Japanese Black male x Holstein female). / Ebara, Fumio; Inada, Sunao; Asaoka, So Hei; Isozaki, Yoshihiro; Saito, Akira; Etoh, Tetsuji; Shiotsuka, Yuji; Gotoh, Takafumi.

In: Journal of Animal and Veterinary Advances, Vol. 9, No. 6, 21.07.2010, p. 982-989.

Research output: Contribution to journalArticle

Ebara, Fumio ; Inada, Sunao ; Asaoka, So Hei ; Isozaki, Yoshihiro ; Saito, Akira ; Etoh, Tetsuji ; Shiotsuka, Yuji ; Gotoh, Takafumi. / Intensive nursing and feeding during the early growth period altered intramuscular adipogenesis in crossbred steers (Japanese Black male x Holstein female). In: Journal of Animal and Veterinary Advances. 2010 ; Vol. 9, No. 6. pp. 982-989.
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AU - Isozaki, Yoshihiro

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AU - Etoh, Tetsuji

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