Interaction between epidermal growth factor and triamcinolone acetonide in mouse palatal mesenchymal cells in vitro

T. Kukita, K. Kurisu

Research output: Contribution to journalArticlepeer-review

Abstract

Palatal mesenchymal cells from mouse embryos (MEPM cells) had a high number of specific receptors for epidermal growth factor (EGF). At 37 °C, the number of high-affinity receptors (dissociation constant, Kd of 6.0 × 10-10 M) was 2.0 × 105 per cell and of low-affinity receptors (Kd of 2.9 × 10-9 M), 3.8 × 105 per cell. At 2 °C, a single class of receptors (Kd of 4.8 × 10-9 M) was detected at 1.8 × 105 per cell. Triamcinolone acetonide, a potent cleft-palate-inducing agent, slightly inhibited the recovery of 125I-labelled EGF binding capacity in MEPM cells after down regulation of EGF receptors; it did not affect the binding properties of 125I-labelled EGF in these cells.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalArchives of Oral Biology
Volume31
Issue number1
DOIs
Publication statusPublished - 1986

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

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