Palatal mesenchymal cells from mouse embryos (MEPM cells) had a high number of specific receptors for epidermal growth factor (EGF). At 37 °C, the number of high-affinity receptors (dissociation constant, Kd of 6.0 × 10-10 M) was 2.0 × 105 per cell and of low-affinity receptors (Kd of 2.9 × 10-9 M), 3.8 × 105 per cell. At 2 °C, a single class of receptors (Kd of 4.8 × 10-9 M) was detected at 1.8 × 105 per cell. Triamcinolone acetonide, a potent cleft-palate-inducing agent, slightly inhibited the recovery of 125I-labelled EGF binding capacity in MEPM cells after down regulation of EGF receptors; it did not affect the binding properties of 125I-labelled EGF in these cells.
All Science Journal Classification (ASJC) codes
- Cell Biology