Interaction between tachyplesin I, an antimicrobial peptide derived from horseshoe crab, and lipopolysaccharide

Takahiro Kushibiki, Masakatsu Kamiya, Tomoyasu Aizawa, Yasuhiro Kumaki, Takashi Kikukawa, Mineyuki Mizuguchi, Makoto Demura, Shun-Ichiro Kawabata, Keiichi Kawano

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Lipopolysaccharide (LPS) is a major constituent of the outer membrane of Gram-negative bacteria and is the very first site of interactions with antimicrobial peptides (AMPs). In order to gain better insight into the interaction between LPS and AMPs, we determined the structure of tachyplesin I (TP I), an antimicrobial peptide derived from horseshoe crab, in its bound state with LPS and proposed the complex structure of TP I and LPS using a docking program. CD and NMR measurements revealed that binding to LPS slightly extends the two β-strands of TP I and stabilizes the whole structure of TP I. The fluorescence wavelength of an intrinsic tryptophan of TP I and fluorescence quenching in the presence or absence of LPS indicated that a tryptophan residue is incorporated into the hydrophobic environment of LPS. Finally, we succeeded in proposing a structural model for the complex of TP I and LPS by using a docking program. The calculated model structure suggested that the cationic residues of TP I interact with phosphate groups and saccharides of LPS, whereas hydrophobic residues interact with the acyl chains of LPS.

Original languageEnglish
Pages (from-to)527-534
Number of pages8
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1844
Issue number3
DOIs
Publication statusPublished - Mar 1 2014

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Horseshoe Crabs
Lipopolysaccharides
Peptides
Tryptophan
Fluorescence
Tachypleus tridentatus tachyplesin peptide
Structural Models
Model structures
Gram-Negative Bacteria
Quenching
Bacteria
Phosphates
Nuclear magnetic resonance
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Analytical Chemistry
  • Molecular Biology

Cite this

Interaction between tachyplesin I, an antimicrobial peptide derived from horseshoe crab, and lipopolysaccharide. / Kushibiki, Takahiro; Kamiya, Masakatsu; Aizawa, Tomoyasu; Kumaki, Yasuhiro; Kikukawa, Takashi; Mizuguchi, Mineyuki; Demura, Makoto; Kawabata, Shun-Ichiro; Kawano, Keiichi.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1844, No. 3, 01.03.2014, p. 527-534.

Research output: Contribution to journalArticle

Kushibiki, Takahiro ; Kamiya, Masakatsu ; Aizawa, Tomoyasu ; Kumaki, Yasuhiro ; Kikukawa, Takashi ; Mizuguchi, Mineyuki ; Demura, Makoto ; Kawabata, Shun-Ichiro ; Kawano, Keiichi. / Interaction between tachyplesin I, an antimicrobial peptide derived from horseshoe crab, and lipopolysaccharide. In: Biochimica et Biophysica Acta - Proteins and Proteomics. 2014 ; Vol. 1844, No. 3. pp. 527-534.
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