Interaction of hREV1 with three human Y-family DNA polymerases

Eiji Ohashi, Yoshiki Murakumo, Naoko Kanjo, Jun Ichi Akagi, Chikahide Masutani, Fumio Hanaoka, Haruo Ohmori

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

Polκ is one of many DNA polymerases involved in translesion DNA synthesis (TLS). It belongs to the Y-family of polymerases along with Polη, Polι and hREV1. Unlike Polη encoded by the xeroderma pigmentosum variant (XPV) gene, Polκ is unable to bypass UV-induced DNA damage in vitro, but it is able to bypass benzo[a]pyrene (B[a]P)-adducted guanines accurately and efficiently. In an attempt to identify factor(s) targeting Polκ to its cognate DNA lesion(s), we searched for Polκ-interacting proteins by using the yeast two-hybrid assay. We found that Polκ interacts with a C-terminal region of hREV1. Polη and Polι were also found to interact with the same region of hREV1. The interaction between Polκ and hREV1 was confirmed by pull-down and coimmunoprecipitation assays. The C-terminal region of hREV1 is known to interact with hREV7, a non-catalytic subunit of Polζ that is another structurally unrelated TLS enzyme, and we show that Polκ and hREV7 bind to the same C-terminal region of hREV1. Thus, our results suggest that hREV1 plays a pivotal role in the multi-enzyme, multi-step process of translesion DNA synthesis.

Original languageEnglish
Pages (from-to)523-531
Number of pages9
JournalGenes to Cells
Volume9
Issue number6
DOIs
Publication statusPublished - Jun 1 2004
Externally publishedYes

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DNA-Directed DNA Polymerase
DNA
pol Gene Products
pol Genes
Two-Hybrid System Techniques
Benzo(a)pyrene
Guanine
Enzymes
DNA Damage

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Ohashi, E., Murakumo, Y., Kanjo, N., Akagi, J. I., Masutani, C., Hanaoka, F., & Ohmori, H. (2004). Interaction of hREV1 with three human Y-family DNA polymerases. Genes to Cells, 9(6), 523-531. https://doi.org/10.1111/j.1356-9597.2004.00747.x

Interaction of hREV1 with three human Y-family DNA polymerases. / Ohashi, Eiji; Murakumo, Yoshiki; Kanjo, Naoko; Akagi, Jun Ichi; Masutani, Chikahide; Hanaoka, Fumio; Ohmori, Haruo.

In: Genes to Cells, Vol. 9, No. 6, 01.06.2004, p. 523-531.

Research output: Contribution to journalArticle

Ohashi, E, Murakumo, Y, Kanjo, N, Akagi, JI, Masutani, C, Hanaoka, F & Ohmori, H 2004, 'Interaction of hREV1 with three human Y-family DNA polymerases', Genes to Cells, vol. 9, no. 6, pp. 523-531. https://doi.org/10.1111/j.1356-9597.2004.00747.x
Ohashi E, Murakumo Y, Kanjo N, Akagi JI, Masutani C, Hanaoka F et al. Interaction of hREV1 with three human Y-family DNA polymerases. Genes to Cells. 2004 Jun 1;9(6):523-531. https://doi.org/10.1111/j.1356-9597.2004.00747.x
Ohashi, Eiji ; Murakumo, Yoshiki ; Kanjo, Naoko ; Akagi, Jun Ichi ; Masutani, Chikahide ; Hanaoka, Fumio ; Ohmori, Haruo. / Interaction of hREV1 with three human Y-family DNA polymerases. In: Genes to Cells. 2004 ; Vol. 9, No. 6. pp. 523-531.
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