Interaction of poly(ethylene glycol)-conjugated phospholipids with supported lipid membranes and their influence on protein adsorption

Toshihiro Yamamoto, Yuji Teramura, Toru Itagaki, Yusuke Arima, Hiroo Iwata

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We studied real-time interaction between poly(ethylene glycol)-conjugated phospholipids (PEG-lipids) and a supported lipid membrane by surface plasmon resonance (SPR) spectroscopy to understand dynamic behaviors of PEG-lipids on living cell membranes. Supported lipid membranes formed on a hydrophobic surface were employed as a model of living cell membrane. We prepared three kinds of PEG-lipids that carried alkyl chains of different lengths for SPR measurements and also performed fluorescence recovery after photobleaching (FRAP) to study the influence of acyl chain length on dynamics on the supported membrane. PEG-lipids were uniformly anchored to lipid membranes with high fluidity without clustering. Incorporation and dissociation rates of PEG-lipids into supported membranes strongly depended on the length of acyl chains; longer acyl chains reduced the incorporation rate and the dissociation rate of PEG-lipid. Furthermore, protein adsorption experiment with bovine serum albumin indicated that PEG modification prevented the adsorption of bovine serum albumin on such supported membrane.

Original languageEnglish
Pages (from-to)677-684
Number of pages8
JournalScience and Technology of Advanced Materials
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

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Phospholipids
Membrane Lipids
Polyethylene glycols
Proteins
Adsorption
Surface plasmon resonance
Cell membranes
Bovine Serum Albumin
Membranes
Photobleaching
Fluidity
Chain length
Fluorescence
Spectroscopy
Recovery

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

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Interaction of poly(ethylene glycol)-conjugated phospholipids with supported lipid membranes and their influence on protein adsorption. / Yamamoto, Toshihiro; Teramura, Yuji; Itagaki, Toru; Arima, Yusuke; Iwata, Hiroo.

In: Science and Technology of Advanced Materials, Vol. 17, No. 1, 01.01.2016, p. 677-684.

Research output: Contribution to journalArticle

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