Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptide inhibitor siamycin I revealed through synchrotron radiation circular dichroism

Mary K. Phillips-Jones, Simon G. Patching, Shalini Edara, Jiro Nakayama, Rohanah Hussain, Giuliano Siligardi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The suitability of synchrotron radiation circular dichroism spectroscopy (SRCD) for studying interactions between the tricyclic peptide inhibitor siamycin I and the intact FsrC membrane sensor kinase in detergent micelles has been established. In the present study, tertiary structural changes demonstrate that inhibitor binding occurs at a different, non-overlapping site to the native ligand, GBAP.

Original languageEnglish
Pages (from-to)444-447
Number of pages4
JournalPhysical Chemistry Chemical Physics
Volume15
Issue number2
DOIs
Publication statusPublished - Jan 14 2013

Fingerprint

Circular dichroism spectroscopy
histidine
Micelles
Synchrotron radiation
Histidine
Detergents
inhibitors
dichroism
peptides
synchrotron radiation
Phosphotransferases
Ligands
membranes
Membranes
Peptides
detergents
Sensors
micelles
interactions
ligands

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

Cite this

Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptide inhibitor siamycin I revealed through synchrotron radiation circular dichroism. / Phillips-Jones, Mary K.; Patching, Simon G.; Edara, Shalini; Nakayama, Jiro; Hussain, Rohanah; Siligardi, Giuliano.

In: Physical Chemistry Chemical Physics, Vol. 15, No. 2, 14.01.2013, p. 444-447.

Research output: Contribution to journalArticle

Phillips-Jones, Mary K. ; Patching, Simon G. ; Edara, Shalini ; Nakayama, Jiro ; Hussain, Rohanah ; Siligardi, Giuliano. / Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptide inhibitor siamycin I revealed through synchrotron radiation circular dichroism. In: Physical Chemistry Chemical Physics. 2013 ; Vol. 15, No. 2. pp. 444-447.
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