Intercellular action of nitric oxide increases cGMP in cerebellar Purkinje cells

Nick A. Hartell, Shigeki Furuya, Sonja Jacoby, Daisuke Okada

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

cGMP is thought to play a role in cerebellar signalling yet its production within Purkinje cells has never been detected. In the present study, the hydrolysis of a fluorescent substrate analogue, 2′-O-anthranyloyl cyclic GMP, by type 5 phosphodiesterase was monitored within Purkinje cells in slices and in culture. Nitric oxide, either endogenous released from adjacent neurons or pharmacologically applied, accelerated the rate of hydrolysis in a manner that was dependent on soluble guanylyl cyclase, demonstrating that nitric oxide triggers cyclic GMP production in Purkinje cells, which in turn activates type 5 phosphodiesterase. We conclude that NO acts as an intercellular messenger in the cerebellar cortex and that parallel fibre terminals are a probable source of nitric oxide.

Original languageEnglish
Pages (from-to)25-28
Number of pages4
JournalNeuroReport
Volume12
Issue number1
DOIs
Publication statusPublished - Jan 22 2001
Externally publishedYes

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Purkinje Cells
Type 5 Cyclic Nucleotide Phosphodiesterases
Nitric Oxide
Cyclic GMP
Hydrolysis
Cerebellar Cortex
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Intercellular action of nitric oxide increases cGMP in cerebellar Purkinje cells. / Hartell, Nick A.; Furuya, Shigeki; Jacoby, Sonja; Okada, Daisuke.

In: NeuroReport, Vol. 12, No. 1, 22.01.2001, p. 25-28.

Research output: Contribution to journalArticle

Hartell, Nick A. ; Furuya, Shigeki ; Jacoby, Sonja ; Okada, Daisuke. / Intercellular action of nitric oxide increases cGMP in cerebellar Purkinje cells. In: NeuroReport. 2001 ; Vol. 12, No. 1. pp. 25-28.
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