Interepithelial signaling with nephric duct is required for the formation of overlying coelomic epithelial cell sheet

Takashi Yoshino, Daisuke Saito, Yuji Atsuta, Chihiro Uchiyama, Shinya Ueda, Kiyotoshi Sekiguchi, Yoshiko Takahashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In most organs of the body, epithelial tissues are supported by their own basement membrane and underlying stroma, the latter being regarded as a complex of amorphous cells, extracellular matrices, and soluble factors. We demonstrate here that an epithelial tube can serve as a component of stroma that supports the formation of epithelial cell sheet derived from a different origin. During development of the mesonephros in chicken embryos, the intermediate mesoderm (IMM), which contains the Wolffian duct (WD) and its associated tubules, is overlain by a sheet of epithelial cells derived from lateral plate (coelomic) mesoderm. We describe that in normal embryos, epitheliogenesis of IMM tubes and the adjacent coelomic cell sheet proceed in a coordinated manner. When the WD was surgically ablated, the overlying coelomic epithelium exhibited aberrant morphology accompanied by a punctated basement membrane. Furthermore, the WD-ablated coelomic epithelium became susceptible to latent external stress; electroporation of Rac1 resulted in epithelial-to-mesenchymal transitions (EMTs) within the coelomic epithelium. The distorted coelomic epithelium was rescued by implanting fibronectin-producing cells in place of the WD, suggesting that fibronectin provided by WD has an important role acting interepithelially. This notion was corroborated further by directly visualizing a translocation of EGFP-tagged fibronectin from fibronectinproducing to -receiving epithelia in vivo. Our findings provide a novel insight into interepithelial signaling that also might occur in adult tissues to protect against EMT and suggest a possible new target for anticancer therapeutic strategy.

Original languageEnglish
Pages (from-to)6660-6665
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number18
DOIs
Publication statusPublished - May 6 2014
Externally publishedYes

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Wolffian Ducts
Epithelium
Epithelial Cells
Kidney
Mesoderm
Fibronectins
Epithelial-Mesenchymal Transition
Basement Membrane
Embryonic Structures
Mesonephros
Electroporation
Extracellular Matrix
Chickens

All Science Journal Classification (ASJC) codes

  • General

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Interepithelial signaling with nephric duct is required for the formation of overlying coelomic epithelial cell sheet. / Yoshino, Takashi; Saito, Daisuke; Atsuta, Yuji; Uchiyama, Chihiro; Ueda, Shinya; Sekiguchi, Kiyotoshi; Takahashi, Yoshiko.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 18, 06.05.2014, p. 6660-6665.

Research output: Contribution to journalArticle

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