Interferons suppress mitochondrial gene transcription by depleting mitochondrial transcription factor A (mtTFA)

Hidetoshi Inagaki, Yuichi Matsushima, Mikiko Ohshima, Yasuo Kitagawa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Mitochondrial gene transcription activity in organello was suppressed after culturing HeLa cells with 1000 U/ml of interferon-α (IFN-α) or IFN- γ for 18 h. The suppression was associated with reduced levels of mitochondrial gene transcripts. Northern blot analysis of HeLa cell RNA showed marked reduction of the mRNA encoding for mitochondrial transcription factor A (mtTFA). Immunoblotting with antiserum directed against recombinant mtTFA showed a reduced level of the protein as well. Consistently, gel- retardation assay of mitochondrial extract showed reduced level of functional mtTFA, which is known to bind to both heavy and light strand promoters of bidirectionally transcribed mitochondrial DNA. We suggest that depletion of mtTFA is a pathway through which IFNs depress the mitochondrial respiration.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalJournal of Interferon and Cytokine Research
Volume17
Issue number5
DOIs
Publication statusPublished - May 1997

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cell Biology
  • Virology

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