Interferons suppress mitochondrial gene transcription by depleting mitochondrial transcription factor A (mtTFA)

Hidetoshi Inagaki, Yuichi Matsushima, Mikiko Ohshima, Yasuo Kitagawa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Mitochondrial gene transcription activity in organello was suppressed after culturing HeLa cells with 1000 U/ml of interferon-α (IFN-α) or IFN- γ for 18 h. The suppression was associated with reduced levels of mitochondrial gene transcripts. Northern blot analysis of HeLa cell RNA showed marked reduction of the mRNA encoding for mitochondrial transcription factor A (mtTFA). Immunoblotting with antiserum directed against recombinant mtTFA showed a reduced level of the protein as well. Consistently, gel- retardation assay of mitochondrial extract showed reduced level of functional mtTFA, which is known to bind to both heavy and light strand promoters of bidirectionally transcribed mitochondrial DNA. We suggest that depletion of mtTFA is a pathway through which IFNs depress the mitochondrial respiration.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalJournal of Interferon and Cytokine Research
Volume17
Issue number5
Publication statusPublished - May 1 1997

Fingerprint

Mitochondrial Genes
Interferons
HeLa Cells
Electrophoretic Mobility Shift Assay
Mitochondrial DNA
Immunoblotting
Northern Blotting
Immune Sera
Respiration
RNA
Messenger RNA
mitochondrial transcription factor A
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cell Biology
  • Virology

Cite this

Interferons suppress mitochondrial gene transcription by depleting mitochondrial transcription factor A (mtTFA). / Inagaki, Hidetoshi; Matsushima, Yuichi; Ohshima, Mikiko; Kitagawa, Yasuo.

In: Journal of Interferon and Cytokine Research, Vol. 17, No. 5, 01.05.1997, p. 263-269.

Research output: Contribution to journalArticle

@article{11aa08d87e264a888eeb6bf45eef891f,
title = "Interferons suppress mitochondrial gene transcription by depleting mitochondrial transcription factor A (mtTFA)",
abstract = "Mitochondrial gene transcription activity in organello was suppressed after culturing HeLa cells with 1000 U/ml of interferon-α (IFN-α) or IFN- γ for 18 h. The suppression was associated with reduced levels of mitochondrial gene transcripts. Northern blot analysis of HeLa cell RNA showed marked reduction of the mRNA encoding for mitochondrial transcription factor A (mtTFA). Immunoblotting with antiserum directed against recombinant mtTFA showed a reduced level of the protein as well. Consistently, gel- retardation assay of mitochondrial extract showed reduced level of functional mtTFA, which is known to bind to both heavy and light strand promoters of bidirectionally transcribed mitochondrial DNA. We suggest that depletion of mtTFA is a pathway through which IFNs depress the mitochondrial respiration.",
author = "Hidetoshi Inagaki and Yuichi Matsushima and Mikiko Ohshima and Yasuo Kitagawa",
year = "1997",
month = "5",
day = "1",
language = "English",
volume = "17",
pages = "263--269",
journal = "Journal of Interferon and Cytokine Research",
issn = "1079-9907",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

TY - JOUR

T1 - Interferons suppress mitochondrial gene transcription by depleting mitochondrial transcription factor A (mtTFA)

AU - Inagaki, Hidetoshi

AU - Matsushima, Yuichi

AU - Ohshima, Mikiko

AU - Kitagawa, Yasuo

PY - 1997/5/1

Y1 - 1997/5/1

N2 - Mitochondrial gene transcription activity in organello was suppressed after culturing HeLa cells with 1000 U/ml of interferon-α (IFN-α) or IFN- γ for 18 h. The suppression was associated with reduced levels of mitochondrial gene transcripts. Northern blot analysis of HeLa cell RNA showed marked reduction of the mRNA encoding for mitochondrial transcription factor A (mtTFA). Immunoblotting with antiserum directed against recombinant mtTFA showed a reduced level of the protein as well. Consistently, gel- retardation assay of mitochondrial extract showed reduced level of functional mtTFA, which is known to bind to both heavy and light strand promoters of bidirectionally transcribed mitochondrial DNA. We suggest that depletion of mtTFA is a pathway through which IFNs depress the mitochondrial respiration.

AB - Mitochondrial gene transcription activity in organello was suppressed after culturing HeLa cells with 1000 U/ml of interferon-α (IFN-α) or IFN- γ for 18 h. The suppression was associated with reduced levels of mitochondrial gene transcripts. Northern blot analysis of HeLa cell RNA showed marked reduction of the mRNA encoding for mitochondrial transcription factor A (mtTFA). Immunoblotting with antiserum directed against recombinant mtTFA showed a reduced level of the protein as well. Consistently, gel- retardation assay of mitochondrial extract showed reduced level of functional mtTFA, which is known to bind to both heavy and light strand promoters of bidirectionally transcribed mitochondrial DNA. We suggest that depletion of mtTFA is a pathway through which IFNs depress the mitochondrial respiration.

UR - http://www.scopus.com/inward/record.url?scp=0030976269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030976269&partnerID=8YFLogxK

M3 - Article

C2 - 9181464

AN - SCOPUS:0030976269

VL - 17

SP - 263

EP - 269

JO - Journal of Interferon and Cytokine Research

JF - Journal of Interferon and Cytokine Research

SN - 1079-9907

IS - 5

ER -