Interleukin-1α activates an NF-κB-like factor in osteoclast-like cells

Eijiro Jimi, Tetsuro Ikebe, Naoyuki Takahashi, Masato Hirata, Tatsuo Suda, Toshitaka Koga

Research output: Contribution to journalArticle

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Abstract

We investigated the NF-κB transcription factor in osteoclast-like cells. Osteoclast-like cells were differentiated from mouse bone marrow cells in co- culture with mouse calvaria-derived primary osteoblasts in the presence of 1α,25-dihydroxyvitamin D3 and prostaglandin E2 in collagen gel-coated dishes. We enriched osteoclast-like cells from the co-cultures by Pronase treatment. When the enriched osteoclast-like cells were treated with phorbol 12-myristate 13-acetate, interleukin-1 (IL-1), calcitonin, or macrophage colony-stimulating factor, only IL-1 activated an NF-κB-like factor, which specifically bound to a κB motif DNA sequence, as detected by an electrophoretic mobility shift assay. IL-1 also activated NF-κB induction in osteoblasts. However, the NF-κB-like factor induced by IL-1-stimulated osteoclast-like cells is of smaller molecular size than the factor in osteoblasts, as shown by an electrophoretic mobility shift assay. The NF-κB activity of osteoclast-like cells was recognized completely by antibodies against the p50 subunit, and only partially by antibodies against the p65 subunit of NF-κB. Antibodies against c-Rel, Rel B, and p52 did not recognize the NF-κB-like factor. These results suggest that IL-1 activates an NF-κB- like factor in osteoclast-like cells, which contains p50 and p65-related proteins.

Original languageEnglish
Pages (from-to)4605-4608
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number9
DOIs
Publication statusPublished - Mar 1 1996

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Osteoclasts
Interleukin-1
Osteoblasts
Electrophoretic mobility
Electrophoretic Mobility Shift Assay
Coculture Techniques
Antibodies
Assays
Cells
Pronase
Macrophage Colony-Stimulating Factor
Calcitriol
DNA sequences
Calcitonin
3'-(1-butylphosphoryl)adenosine
Cell culture
Dinoprostone
Skull
Bone Marrow Cells
Bone

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Interleukin-1α activates an NF-κB-like factor in osteoclast-like cells. / Jimi, Eijiro; Ikebe, Tetsuro; Takahashi, Naoyuki; Hirata, Masato; Suda, Tatsuo; Koga, Toshitaka.

In: Journal of Biological Chemistry, Vol. 271, No. 9, 01.03.1996, p. 4605-4608.

Research output: Contribution to journalArticle

Jimi, E, Ikebe, T, Takahashi, N, Hirata, M, Suda, T & Koga, T 1996, 'Interleukin-1α activates an NF-κB-like factor in osteoclast-like cells', Journal of Biological Chemistry, vol. 271, no. 9, pp. 4605-4608. https://doi.org/10.1074/jbc.271.9.4605
Jimi, Eijiro ; Ikebe, Tetsuro ; Takahashi, Naoyuki ; Hirata, Masato ; Suda, Tatsuo ; Koga, Toshitaka. / Interleukin-1α activates an NF-κB-like factor in osteoclast-like cells. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 9. pp. 4605-4608.
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