Interleukin-10 from transplanted bone marrow mononuclear cells contributes to cardiac protection after myocardial infarction

Jana S. Burchfield, Masayoshi Iwasaki, Masamichi Koyanagi, Carmen Urbich, Nadia Rosenthal, Andreas M. Zeiher, Stefanie Dimmeler

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Bone marrow mononuclear cells (BM-MNCs) have successfully been used as a therapy for the improvement of left ventricular (LV) function after myocardial infarction (MI). It has been suggested that paracrine factors from BM-MNCs may be a key mechanism mediating cardiac protection. We previously performed microarray analysis and found that the pleiotropic cytokine interleukin (IL)-10 was highly upregulated in human progenitor cells in comparison with adult endothelial cells and CD14 cells. Moreover, BM-MNCs secrete significant amounts of IL-10, and IL-10 could be detected from progenitor cells transplanted in infarcted mouse hearts. Specifically, intramyocardial injection of wild-type BM-MNCs led to a significant decrease in LV end-diastolic pressure (LVEDP) and LV end-systolic volume (LVESV) compared to hearts injected with either diluent or IL-10 knock-out BM-MNCs. Furthermore, intramyocardial injection of wild-type BM-MNCs led to a significant increase in stroke volume (SV) and rate of the development of pressure over time (+dP/dt) compared to hearts injected with either diluent or IL-10 knock-out BM-MNCs. The IL-10-dependent improvement provided by transplanted cells was not caused by reduced infarct size, neutrophil infiltration, or capillary density, but rather was associated with decreased T lymphocyte accumulation, reactive hypertrophy, and myocardial collagen deposition. These results suggest that BM-MNCs mediate cardiac protection after myocardial infarction and this is, at least in part, dependent on IL-10.

Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalCirculation research
Volume103
Issue number2
DOIs
Publication statusPublished - Jul 18 2008

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Bone Marrow Cells
Interleukin-10
Myocardial Infarction
Stroke Volume
Stem Cells
Injections
Neutrophil Infiltration
Microarray Analysis
Left Ventricular Function
Hypertrophy
Collagen
Endothelial Cells
Cytokines
Blood Pressure
T-Lymphocytes
Pressure

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Burchfield, J. S., Iwasaki, M., Koyanagi, M., Urbich, C., Rosenthal, N., Zeiher, A. M., & Dimmeler, S. (2008). Interleukin-10 from transplanted bone marrow mononuclear cells contributes to cardiac protection after myocardial infarction. Circulation research, 103(2), 203-211. https://doi.org/10.1161/CIRCRESAHA.108.178475

Interleukin-10 from transplanted bone marrow mononuclear cells contributes to cardiac protection after myocardial infarction. / Burchfield, Jana S.; Iwasaki, Masayoshi; Koyanagi, Masamichi; Urbich, Carmen; Rosenthal, Nadia; Zeiher, Andreas M.; Dimmeler, Stefanie.

In: Circulation research, Vol. 103, No. 2, 18.07.2008, p. 203-211.

Research output: Contribution to journalArticle

Burchfield, JS, Iwasaki, M, Koyanagi, M, Urbich, C, Rosenthal, N, Zeiher, AM & Dimmeler, S 2008, 'Interleukin-10 from transplanted bone marrow mononuclear cells contributes to cardiac protection after myocardial infarction', Circulation research, vol. 103, no. 2, pp. 203-211. https://doi.org/10.1161/CIRCRESAHA.108.178475
Burchfield, Jana S. ; Iwasaki, Masayoshi ; Koyanagi, Masamichi ; Urbich, Carmen ; Rosenthal, Nadia ; Zeiher, Andreas M. ; Dimmeler, Stefanie. / Interleukin-10 from transplanted bone marrow mononuclear cells contributes to cardiac protection after myocardial infarction. In: Circulation research. 2008 ; Vol. 103, No. 2. pp. 203-211.
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