Interleukin 15 activity in the rectal mucosa of inflammatory bowel disease

T. Sakai, K. Kusugami, H. Nishimura, T. Ando, T. Yamaguchi, M. Ohsuga, K. Ina, A. Enomoto, Y. Kimura, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    90 Citations (Scopus)

    Abstract

    Background and Aims: Interleukin (IL)-15 has been found to share many immunoregulatory activities in lymphocytes with IL-2. The aim of this study was to investigate IL-15 activity in organ cultures, localization of IL-15 messenger RNA (mRNA), and proliferation of lamina propria mononuclear cells (LPMCs) in response to recombinant IL-15 using the mucosal tissues obtained from patients with inflammatory bowel disease (IBD). Methods: The contents of IL-15, tumor necrosis factor α, and IL-2 in the culture supernatant of the rectal mucosal tissues were determined by an enzyme-linked immunosorbent assay. Expression of IL-15 mRNA was analyzed by in situ hybridization, and proliferative response of LPMCs to recombinant IL-15 was determined by [3H]thymidine incorporation into DNA. Results: Significantly greater IL-15 activity was detected in active IBD, and this elevation was also observed in inactive ulcerative colitis. In contrast, greater tumor necrosis factor α activity was observed only in active IBD, and IL-2 was not detected in organ cultures. In situ hybridization showed IL-15 mRNA in macrophages and epithelial cells in active IBD specimens, and recombinant IL-15 induced a dose-dependent proliferative response in LPMCs. Conclusions: Mucosal IL-15 may be involved in the pathogenesis of IBD as one of the important mediators in activation of mucosal immune cells.

    Original languageEnglish
    Pages (from-to)1237-1243
    Number of pages7
    JournalGastroenterology
    Volume114
    Issue number6
    DOIs
    Publication statusPublished - Jan 1 1998

    Fingerprint

    Interleukin-15
    Inflammatory Bowel Diseases
    Mucous Membrane
    Interleukin-2
    Organ Culture Techniques
    Messenger RNA
    In Situ Hybridization
    Tumor Necrosis Factor-alpha
    Ulcerative Colitis
    Thymidine
    Epithelial Cells
    Enzyme-Linked Immunosorbent Assay
    Macrophages
    Lymphocytes

    All Science Journal Classification (ASJC) codes

    • Hepatology
    • Gastroenterology

    Cite this

    Sakai, T., Kusugami, K., Nishimura, H., Ando, T., Yamaguchi, T., Ohsuga, M., ... Yoshikai, Y. (1998). Interleukin 15 activity in the rectal mucosa of inflammatory bowel disease. Gastroenterology, 114(6), 1237-1243. https://doi.org/10.1016/S0016-5085(98)70430-5

    Interleukin 15 activity in the rectal mucosa of inflammatory bowel disease. / Sakai, T.; Kusugami, K.; Nishimura, H.; Ando, T.; Yamaguchi, T.; Ohsuga, M.; Ina, K.; Enomoto, A.; Kimura, Y.; Yoshikai, Yasunobu.

    In: Gastroenterology, Vol. 114, No. 6, 01.01.1998, p. 1237-1243.

    Research output: Contribution to journalArticle

    Sakai, T, Kusugami, K, Nishimura, H, Ando, T, Yamaguchi, T, Ohsuga, M, Ina, K, Enomoto, A, Kimura, Y & Yoshikai, Y 1998, 'Interleukin 15 activity in the rectal mucosa of inflammatory bowel disease', Gastroenterology, vol. 114, no. 6, pp. 1237-1243. https://doi.org/10.1016/S0016-5085(98)70430-5
    Sakai T, Kusugami K, Nishimura H, Ando T, Yamaguchi T, Ohsuga M et al. Interleukin 15 activity in the rectal mucosa of inflammatory bowel disease. Gastroenterology. 1998 Jan 1;114(6):1237-1243. https://doi.org/10.1016/S0016-5085(98)70430-5
    Sakai, T. ; Kusugami, K. ; Nishimura, H. ; Ando, T. ; Yamaguchi, T. ; Ohsuga, M. ; Ina, K. ; Enomoto, A. ; Kimura, Y. ; Yoshikai, Yasunobu. / Interleukin 15 activity in the rectal mucosa of inflammatory bowel disease. In: Gastroenterology. 1998 ; Vol. 114, No. 6. pp. 1237-1243.
    @article{f04b4ec400e34859995eb7e8e58b0de8,
    title = "Interleukin 15 activity in the rectal mucosa of inflammatory bowel disease",
    abstract = "Background and Aims: Interleukin (IL)-15 has been found to share many immunoregulatory activities in lymphocytes with IL-2. The aim of this study was to investigate IL-15 activity in organ cultures, localization of IL-15 messenger RNA (mRNA), and proliferation of lamina propria mononuclear cells (LPMCs) in response to recombinant IL-15 using the mucosal tissues obtained from patients with inflammatory bowel disease (IBD). Methods: The contents of IL-15, tumor necrosis factor α, and IL-2 in the culture supernatant of the rectal mucosal tissues were determined by an enzyme-linked immunosorbent assay. Expression of IL-15 mRNA was analyzed by in situ hybridization, and proliferative response of LPMCs to recombinant IL-15 was determined by [3H]thymidine incorporation into DNA. Results: Significantly greater IL-15 activity was detected in active IBD, and this elevation was also observed in inactive ulcerative colitis. In contrast, greater tumor necrosis factor α activity was observed only in active IBD, and IL-2 was not detected in organ cultures. In situ hybridization showed IL-15 mRNA in macrophages and epithelial cells in active IBD specimens, and recombinant IL-15 induced a dose-dependent proliferative response in LPMCs. Conclusions: Mucosal IL-15 may be involved in the pathogenesis of IBD as one of the important mediators in activation of mucosal immune cells.",
    author = "T. Sakai and K. Kusugami and H. Nishimura and T. Ando and T. Yamaguchi and M. Ohsuga and K. Ina and A. Enomoto and Y. Kimura and Yasunobu Yoshikai",
    year = "1998",
    month = "1",
    day = "1",
    doi = "10.1016/S0016-5085(98)70430-5",
    language = "English",
    volume = "114",
    pages = "1237--1243",
    journal = "Gastroenterology",
    issn = "0016-5085",
    publisher = "W.B. Saunders Ltd",
    number = "6",

    }

    TY - JOUR

    T1 - Interleukin 15 activity in the rectal mucosa of inflammatory bowel disease

    AU - Sakai, T.

    AU - Kusugami, K.

    AU - Nishimura, H.

    AU - Ando, T.

    AU - Yamaguchi, T.

    AU - Ohsuga, M.

    AU - Ina, K.

    AU - Enomoto, A.

    AU - Kimura, Y.

    AU - Yoshikai, Yasunobu

    PY - 1998/1/1

    Y1 - 1998/1/1

    N2 - Background and Aims: Interleukin (IL)-15 has been found to share many immunoregulatory activities in lymphocytes with IL-2. The aim of this study was to investigate IL-15 activity in organ cultures, localization of IL-15 messenger RNA (mRNA), and proliferation of lamina propria mononuclear cells (LPMCs) in response to recombinant IL-15 using the mucosal tissues obtained from patients with inflammatory bowel disease (IBD). Methods: The contents of IL-15, tumor necrosis factor α, and IL-2 in the culture supernatant of the rectal mucosal tissues were determined by an enzyme-linked immunosorbent assay. Expression of IL-15 mRNA was analyzed by in situ hybridization, and proliferative response of LPMCs to recombinant IL-15 was determined by [3H]thymidine incorporation into DNA. Results: Significantly greater IL-15 activity was detected in active IBD, and this elevation was also observed in inactive ulcerative colitis. In contrast, greater tumor necrosis factor α activity was observed only in active IBD, and IL-2 was not detected in organ cultures. In situ hybridization showed IL-15 mRNA in macrophages and epithelial cells in active IBD specimens, and recombinant IL-15 induced a dose-dependent proliferative response in LPMCs. Conclusions: Mucosal IL-15 may be involved in the pathogenesis of IBD as one of the important mediators in activation of mucosal immune cells.

    AB - Background and Aims: Interleukin (IL)-15 has been found to share many immunoregulatory activities in lymphocytes with IL-2. The aim of this study was to investigate IL-15 activity in organ cultures, localization of IL-15 messenger RNA (mRNA), and proliferation of lamina propria mononuclear cells (LPMCs) in response to recombinant IL-15 using the mucosal tissues obtained from patients with inflammatory bowel disease (IBD). Methods: The contents of IL-15, tumor necrosis factor α, and IL-2 in the culture supernatant of the rectal mucosal tissues were determined by an enzyme-linked immunosorbent assay. Expression of IL-15 mRNA was analyzed by in situ hybridization, and proliferative response of LPMCs to recombinant IL-15 was determined by [3H]thymidine incorporation into DNA. Results: Significantly greater IL-15 activity was detected in active IBD, and this elevation was also observed in inactive ulcerative colitis. In contrast, greater tumor necrosis factor α activity was observed only in active IBD, and IL-2 was not detected in organ cultures. In situ hybridization showed IL-15 mRNA in macrophages and epithelial cells in active IBD specimens, and recombinant IL-15 induced a dose-dependent proliferative response in LPMCs. Conclusions: Mucosal IL-15 may be involved in the pathogenesis of IBD as one of the important mediators in activation of mucosal immune cells.

    UR - http://www.scopus.com/inward/record.url?scp=0031750280&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0031750280&partnerID=8YFLogxK

    U2 - 10.1016/S0016-5085(98)70430-5

    DO - 10.1016/S0016-5085(98)70430-5

    M3 - Article

    VL - 114

    SP - 1237

    EP - 1243

    JO - Gastroenterology

    JF - Gastroenterology

    SN - 0016-5085

    IS - 6

    ER -