Interleukin-15 may be responsible for early activation of intestinal intraepithelial lymphocytes after oral infection with Listeria monocytogenes in rats

Kenji Hirose, Hirohiko Suzuki, Hitoshi Nishimura, Akio Mitani, Junji Washizu, Tetsuya Matsuguchi, Yasunobu Yoshikai

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    Abstract

    Exogenous interleukin-15 (IL-15) stimulates intestinal intraepithelial lymphocytes (i-IEL) from mice to proliferate and produce gamma interferon (IFN-γ) in vitro. To determine whether endogenous IL-15 is involved in activation of i-IEL during intestinal infection, we examined IL-15 synthesis by intestinal epithelial cells (i-EC) after infection with Listeria monocytogenes in rats. In in vitro experiments, invasion of L. monocytogenes into IEC-6 cells, a rat small intestine epithelial cell line, evidently induced IL-15 mRNA expression coincident with nuclear factor κB (NF-κB) activation, which is essential for IL-15 gene expression. IL-15 synthesis was detected in rat i-EC on day 1 after an oral inoculation of L. monocytogenes in vivo. The numbers of T-cell receptor (TCR) γδ+ T cells, NKR.P1+ cells, and CD3+ CD8+ αα cells in i-IEL were significantly increased on day 1 after oral infection. The i-IEL from infected rats produced larger amounts of IFN-γ upon stimulation with immobilized anti-TCR γδ or anti-NKR.P1 monoclonal antibodies. These results suggest that IL-15 produced by i-EC may stimulate significant fractions of i-IEL to produce IFN-γ at an early phase of oral infection with L. monocytogenes.

    Original languageEnglish
    Pages (from-to)5677-5683
    Number of pages7
    JournalInfection and Immunity
    Volume66
    Issue number12
    Publication statusPublished - Dec 1 1998

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    All Science Journal Classification (ASJC) codes

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases

    Cite this

    Hirose, K., Suzuki, H., Nishimura, H., Mitani, A., Washizu, J., Matsuguchi, T., & Yoshikai, Y. (1998). Interleukin-15 may be responsible for early activation of intestinal intraepithelial lymphocytes after oral infection with Listeria monocytogenes in rats. Infection and Immunity, 66(12), 5677-5683.