Interleukin-15 may be responsible for early activation of intestinal intraepithelial lymphocytes after oral infection with Listeria monocytogenes in rats

Kenji Hirose, Hirohiko Suzuki, Hitoshi Nishimura, Akio Mitani, Junji Washizu, Tetsuya Matsuguchi, Yasunobu Yoshikai

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Abstract

Exogenous interleukin-15 (IL-15) stimulates intestinal intraepithelial lymphocytes (i-IEL) from mice to proliferate and produce gamma interferon (IFN-γ) in vitro. To determine whether endogenous IL-15 is involved in activation of i-IEL during intestinal infection, we examined IL-15 synthesis by intestinal epithelial cells (i-EC) after infection with Listeria monocytogenes in rats. In in vitro experiments, invasion of L. monocytogenes into IEC-6 cells, a rat small intestine epithelial cell line, evidently induced IL-15 mRNA expression coincident with nuclear factor κB (NF-κB) activation, which is essential for IL-15 gene expression. IL-15 synthesis was detected in rat i-EC on day 1 after an oral inoculation of L. monocytogenes in vivo. The numbers of T-cell receptor (TCR) γδ+ T cells, NKR.P1+ cells, and CD3+ CD8+ αα cells in i-IEL were significantly increased on day 1 after oral infection. The i-IEL from infected rats produced larger amounts of IFN-γ upon stimulation with immobilized anti-TCR γδ or anti-NKR.P1 monoclonal antibodies. These results suggest that IL-15 produced by i-EC may stimulate significant fractions of i-IEL to produce IFN-γ at an early phase of oral infection with L. monocytogenes.

Original languageEnglish
Pages (from-to)5677-5683
Number of pages7
JournalInfection and Immunity
Volume66
Issue number12
Publication statusPublished - Dec 1998
Externally publishedYes

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Interleukin-15
Listeria monocytogenes
Lymphocytes
Infection
Epithelial Cells
T-Cell Antigen Receptor
Interferons
Interferon-gamma
Small Intestine
Monoclonal Antibodies
T-Lymphocytes
Gene Expression
Cell Line
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Hirose, K., Suzuki, H., Nishimura, H., Mitani, A., Washizu, J., Matsuguchi, T., & Yoshikai, Y. (1998). Interleukin-15 may be responsible for early activation of intestinal intraepithelial lymphocytes after oral infection with Listeria monocytogenes in rats. Infection and Immunity, 66(12), 5677-5683.

Interleukin-15 may be responsible for early activation of intestinal intraepithelial lymphocytes after oral infection with Listeria monocytogenes in rats. / Hirose, Kenji; Suzuki, Hirohiko; Nishimura, Hitoshi; Mitani, Akio; Washizu, Junji; Matsuguchi, Tetsuya; Yoshikai, Yasunobu.

In: Infection and Immunity, Vol. 66, No. 12, 12.1998, p. 5677-5683.

Research output: Contribution to journalArticle

Hirose, K, Suzuki, H, Nishimura, H, Mitani, A, Washizu, J, Matsuguchi, T & Yoshikai, Y 1998, 'Interleukin-15 may be responsible for early activation of intestinal intraepithelial lymphocytes after oral infection with Listeria monocytogenes in rats', Infection and Immunity, vol. 66, no. 12, pp. 5677-5683.
Hirose, Kenji ; Suzuki, Hirohiko ; Nishimura, Hitoshi ; Mitani, Akio ; Washizu, Junji ; Matsuguchi, Tetsuya ; Yoshikai, Yasunobu. / Interleukin-15 may be responsible for early activation of intestinal intraepithelial lymphocytes after oral infection with Listeria monocytogenes in rats. In: Infection and Immunity. 1998 ; Vol. 66, No. 12. pp. 5677-5683.
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