Abstract
Non-alcoholic fatty liver disease (NAFLD) is a common cause of chronic non-viral liver disease. Activation of macrophages and hepatic stellate cells is a critical step that promotes liver fibrosis. We aimed to explore the feasibility of interleukin-34 (IL-34), a key regulator of macrophages, as a fibrosis marker in patients with NAFLD. We enrolled 197 liver biopsy-proven NAFLD patients. We evaluated the serum levels of IL-34, macrophage-colony stimulating factor (M-CSF), soluble CD163 (sCD163), 40 cytokines/chemokines, hyaluronic acid, type IV collagen 7s, and clinically-approved fibrosis scores. IL-34 increased with the progression of fibrosis and was an independent marker for liver fibrosis. Immunostaining experiments, using resected liver specimens from NAFLD patients, revealed that IL-34 was mainly expressed on liver fibroblasts. IL-34 based fibrosis score (0.0387∗IL-34 (pg/ml) + 0.3623∗type IV collagen 7s (ng/ml) + 0.0184∗age (year)-1.1850) was a practical predictive model of liver fibrosis. Using receiver-operating characteristic analyses, the area under the curve, sensitivity, and specificity of IL-34 based fibrosis score were superior or comparable to the other fibrosis biomarkers and scores. In conclusion, the IL-34 based fibrosis score, including serum IL-34, type IV collagen 7s and age, is a feasible diagnostic marker of liver fibrosis in NAFLD patients.
| Original language | English |
|---|---|
| Article number | 28814 |
| Journal | Scientific reports |
| Volume | 6 |
| DOIs | |
| Publication status | Published - Jul 1 2016 |
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All Science Journal Classification (ASJC) codes
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Interleukin-34 as a fibroblast-derived marker of liver fibrosis in patients with non-alcoholic fatty liver disease. / Shoji, Hirotaka; Yoshio, Sachiyo; Mano, Yohei; Kumagai, Erina; Sugiyama, Masaya; Korenaga, Masaaki; Arai, Taeang; Itokawa, Norio; Atsukawa, Masanori; Aikata, Hiroshi; Hyogo, Hideyuki; Chayama, Kazuaki; Ohashi, Tomohiko; Ito, Kiyoaki; Yoneda, Masashi; Nozaki, Yuichi; Kawaguchi, Takumi; Torimura, Takuji; Abe, Masanori; Hiasa, Yoichi; Fukai, Moto; Kamiyama, Toshiya; Taketomi, Akinobu; Mizokami, Masashi; Kanto, Tatsuya.
In: Scientific reports, Vol. 6, 28814, 01.07.2016.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Interleukin-34 as a fibroblast-derived marker of liver fibrosis in patients with non-alcoholic fatty liver disease
AU - Shoji, Hirotaka
AU - Yoshio, Sachiyo
AU - Mano, Yohei
AU - Kumagai, Erina
AU - Sugiyama, Masaya
AU - Korenaga, Masaaki
AU - Arai, Taeang
AU - Itokawa, Norio
AU - Atsukawa, Masanori
AU - Aikata, Hiroshi
AU - Hyogo, Hideyuki
AU - Chayama, Kazuaki
AU - Ohashi, Tomohiko
AU - Ito, Kiyoaki
AU - Yoneda, Masashi
AU - Nozaki, Yuichi
AU - Kawaguchi, Takumi
AU - Torimura, Takuji
AU - Abe, Masanori
AU - Hiasa, Yoichi
AU - Fukai, Moto
AU - Kamiyama, Toshiya
AU - Taketomi, Akinobu
AU - Mizokami, Masashi
AU - Kanto, Tatsuya
PY - 2016/7/1
Y1 - 2016/7/1
N2 - Non-alcoholic fatty liver disease (NAFLD) is a common cause of chronic non-viral liver disease. Activation of macrophages and hepatic stellate cells is a critical step that promotes liver fibrosis. We aimed to explore the feasibility of interleukin-34 (IL-34), a key regulator of macrophages, as a fibrosis marker in patients with NAFLD. We enrolled 197 liver biopsy-proven NAFLD patients. We evaluated the serum levels of IL-34, macrophage-colony stimulating factor (M-CSF), soluble CD163 (sCD163), 40 cytokines/chemokines, hyaluronic acid, type IV collagen 7s, and clinically-approved fibrosis scores. IL-34 increased with the progression of fibrosis and was an independent marker for liver fibrosis. Immunostaining experiments, using resected liver specimens from NAFLD patients, revealed that IL-34 was mainly expressed on liver fibroblasts. IL-34 based fibrosis score (0.0387∗IL-34 (pg/ml) + 0.3623∗type IV collagen 7s (ng/ml) + 0.0184∗age (year)-1.1850) was a practical predictive model of liver fibrosis. Using receiver-operating characteristic analyses, the area under the curve, sensitivity, and specificity of IL-34 based fibrosis score were superior or comparable to the other fibrosis biomarkers and scores. In conclusion, the IL-34 based fibrosis score, including serum IL-34, type IV collagen 7s and age, is a feasible diagnostic marker of liver fibrosis in NAFLD patients.
AB - Non-alcoholic fatty liver disease (NAFLD) is a common cause of chronic non-viral liver disease. Activation of macrophages and hepatic stellate cells is a critical step that promotes liver fibrosis. We aimed to explore the feasibility of interleukin-34 (IL-34), a key regulator of macrophages, as a fibrosis marker in patients with NAFLD. We enrolled 197 liver biopsy-proven NAFLD patients. We evaluated the serum levels of IL-34, macrophage-colony stimulating factor (M-CSF), soluble CD163 (sCD163), 40 cytokines/chemokines, hyaluronic acid, type IV collagen 7s, and clinically-approved fibrosis scores. IL-34 increased with the progression of fibrosis and was an independent marker for liver fibrosis. Immunostaining experiments, using resected liver specimens from NAFLD patients, revealed that IL-34 was mainly expressed on liver fibroblasts. IL-34 based fibrosis score (0.0387∗IL-34 (pg/ml) + 0.3623∗type IV collagen 7s (ng/ml) + 0.0184∗age (year)-1.1850) was a practical predictive model of liver fibrosis. Using receiver-operating characteristic analyses, the area under the curve, sensitivity, and specificity of IL-34 based fibrosis score were superior or comparable to the other fibrosis biomarkers and scores. In conclusion, the IL-34 based fibrosis score, including serum IL-34, type IV collagen 7s and age, is a feasible diagnostic marker of liver fibrosis in NAFLD patients.
UR - http://www.scopus.com/inward/record.url?scp=84976871484&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84976871484&partnerID=8YFLogxK
U2 - 10.1038/srep28814
DO - 10.1038/srep28814
M3 - Article
C2 - 27363523
AN - SCOPUS:84976871484
VL - 6
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 28814
ER -