Interleukin-8 and SDF1-α mRNA expression in colonic biopsies from patients with inflammatory bowel disease

Takeshi Katsuta, Christina Lim, Katsuhiro Shimoda, Kenji Shibuta, Prasenjit Mitra, Barbara F. Banner, Masaki Mori, Graham F. Barnard

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

OBJECTIVES: Interleukin-8 (IL-8) as an α-chemokine recruits and activates neutrophils, which are abundant in the intestinal lesions of ulcerative colitis (UC) and Crohn's disease (CD). Stromal cell-derived factor 1 (SDF1-α) is a new chemokine that is chemotactic to neutrophils. The aims of this study were to assess the relative expression of SDF1-α and IL-8 mRNA in different colonic regions and patients with inflammatory bowel disease with varied degrees of inflammation in the colon. METHODS: Colon biopsy samples were obtained from 19 patients with UC, 12 with CD, and 5 with irritable bowel syndrome (IBS) who underwent colonoscopy. Levels of IL-8 and SDF1-α mRNA expression were measured semiquantitatively by reverse-transcription and polymerase chain reaction amplification. The cytokine mRNA levels were corrected for glyceraldelyde-3-phosphate dehydrogenase mRNA expression. RESULTS: IL-8 mRNA expression was significantly correlated with SDF1-α expression in normal biopsies from IBS patients (r = 0.58, p < 0.01). There was no significant difference in cytokine mRNA expression (IL-8 or SDF1-α) across different regions of the colon or rectum in uninflamed normal biopsies. The IL-8 mRNA expression ratios in UC (mean ± SD, 1.03 ± 0.52) and CD (0.90 ± 0.38) patients were significantly higher than in IBS (0.52 ± 0.17) (p <0.01, p < 0.05, respectively). The SDF1-α mRNA expression ratio in UC (0.30 ± 0.52) was higher than in both CD (0.21 ± 0.10) and IBS patients (0.22 ± 0.11) (p < 0.01, <0.05, respectively). A statistically significant correlation was found between the IL-8 mRNA expression and the colonic inflammation in UC patients (r = 0.44, p < 0.05) but not for SDF1-α expression in UC patients. CONCLUSIONS: IL-8 but not SDF1-α mRNA expression was associated with inflammation in UC. This suggests that IL-8 may play a more important role in inflammatory bowel disease than does SDF1-α. (C) 2000 by Am. Coll. of Gastroenterology.

Original languageEnglish
Pages (from-to)3157-3164
Number of pages8
JournalAmerican Journal of Gastroenterology
Volume95
Issue number11
DOIs
Publication statusPublished - Nov 20 2000

Fingerprint

Interleukin-8
Inflammatory Bowel Diseases
Ulcerative Colitis
Biopsy
Messenger RNA
Irritable Bowel Syndrome
Crohn Disease
Colon
Inflammation
Chemokines
Neutrophils
Cytokines
Chemokine CXCL12
Gastroenterology
Colonoscopy
Rectum
Reverse Transcription
Oxidoreductases
Phosphates
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Interleukin-8 and SDF1-α mRNA expression in colonic biopsies from patients with inflammatory bowel disease. / Katsuta, Takeshi; Lim, Christina; Shimoda, Katsuhiro; Shibuta, Kenji; Mitra, Prasenjit; Banner, Barbara F.; Mori, Masaki; Barnard, Graham F.

In: American Journal of Gastroenterology, Vol. 95, No. 11, 20.11.2000, p. 3157-3164.

Research output: Contribution to journalArticle

Katsuta, Takeshi ; Lim, Christina ; Shimoda, Katsuhiro ; Shibuta, Kenji ; Mitra, Prasenjit ; Banner, Barbara F. ; Mori, Masaki ; Barnard, Graham F. / Interleukin-8 and SDF1-α mRNA expression in colonic biopsies from patients with inflammatory bowel disease. In: American Journal of Gastroenterology. 2000 ; Vol. 95, No. 11. pp. 3157-3164.
@article{7209a4aef1ff41c4b0f1ea7358a2541f,
title = "Interleukin-8 and SDF1-α mRNA expression in colonic biopsies from patients with inflammatory bowel disease",
abstract = "OBJECTIVES: Interleukin-8 (IL-8) as an α-chemokine recruits and activates neutrophils, which are abundant in the intestinal lesions of ulcerative colitis (UC) and Crohn's disease (CD). Stromal cell-derived factor 1 (SDF1-α) is a new chemokine that is chemotactic to neutrophils. The aims of this study were to assess the relative expression of SDF1-α and IL-8 mRNA in different colonic regions and patients with inflammatory bowel disease with varied degrees of inflammation in the colon. METHODS: Colon biopsy samples were obtained from 19 patients with UC, 12 with CD, and 5 with irritable bowel syndrome (IBS) who underwent colonoscopy. Levels of IL-8 and SDF1-α mRNA expression were measured semiquantitatively by reverse-transcription and polymerase chain reaction amplification. The cytokine mRNA levels were corrected for glyceraldelyde-3-phosphate dehydrogenase mRNA expression. RESULTS: IL-8 mRNA expression was significantly correlated with SDF1-α expression in normal biopsies from IBS patients (r = 0.58, p < 0.01). There was no significant difference in cytokine mRNA expression (IL-8 or SDF1-α) across different regions of the colon or rectum in uninflamed normal biopsies. The IL-8 mRNA expression ratios in UC (mean ± SD, 1.03 ± 0.52) and CD (0.90 ± 0.38) patients were significantly higher than in IBS (0.52 ± 0.17) (p <0.01, p < 0.05, respectively). The SDF1-α mRNA expression ratio in UC (0.30 ± 0.52) was higher than in both CD (0.21 ± 0.10) and IBS patients (0.22 ± 0.11) (p < 0.01, <0.05, respectively). A statistically significant correlation was found between the IL-8 mRNA expression and the colonic inflammation in UC patients (r = 0.44, p < 0.05) but not for SDF1-α expression in UC patients. CONCLUSIONS: IL-8 but not SDF1-α mRNA expression was associated with inflammation in UC. This suggests that IL-8 may play a more important role in inflammatory bowel disease than does SDF1-α. (C) 2000 by Am. Coll. of Gastroenterology.",
author = "Takeshi Katsuta and Christina Lim and Katsuhiro Shimoda and Kenji Shibuta and Prasenjit Mitra and Banner, {Barbara F.} and Masaki Mori and Barnard, {Graham F.}",
year = "2000",
month = "11",
day = "20",
doi = "10.1016/S0002-9270(00)02082-7",
language = "English",
volume = "95",
pages = "3157--3164",
journal = "American Journal of Gastroenterology",
issn = "0002-9270",
publisher = "Nature Publishing Group",
number = "11",

}

TY - JOUR

T1 - Interleukin-8 and SDF1-α mRNA expression in colonic biopsies from patients with inflammatory bowel disease

AU - Katsuta, Takeshi

AU - Lim, Christina

AU - Shimoda, Katsuhiro

AU - Shibuta, Kenji

AU - Mitra, Prasenjit

AU - Banner, Barbara F.

AU - Mori, Masaki

AU - Barnard, Graham F.

PY - 2000/11/20

Y1 - 2000/11/20

N2 - OBJECTIVES: Interleukin-8 (IL-8) as an α-chemokine recruits and activates neutrophils, which are abundant in the intestinal lesions of ulcerative colitis (UC) and Crohn's disease (CD). Stromal cell-derived factor 1 (SDF1-α) is a new chemokine that is chemotactic to neutrophils. The aims of this study were to assess the relative expression of SDF1-α and IL-8 mRNA in different colonic regions and patients with inflammatory bowel disease with varied degrees of inflammation in the colon. METHODS: Colon biopsy samples were obtained from 19 patients with UC, 12 with CD, and 5 with irritable bowel syndrome (IBS) who underwent colonoscopy. Levels of IL-8 and SDF1-α mRNA expression were measured semiquantitatively by reverse-transcription and polymerase chain reaction amplification. The cytokine mRNA levels were corrected for glyceraldelyde-3-phosphate dehydrogenase mRNA expression. RESULTS: IL-8 mRNA expression was significantly correlated with SDF1-α expression in normal biopsies from IBS patients (r = 0.58, p < 0.01). There was no significant difference in cytokine mRNA expression (IL-8 or SDF1-α) across different regions of the colon or rectum in uninflamed normal biopsies. The IL-8 mRNA expression ratios in UC (mean ± SD, 1.03 ± 0.52) and CD (0.90 ± 0.38) patients were significantly higher than in IBS (0.52 ± 0.17) (p <0.01, p < 0.05, respectively). The SDF1-α mRNA expression ratio in UC (0.30 ± 0.52) was higher than in both CD (0.21 ± 0.10) and IBS patients (0.22 ± 0.11) (p < 0.01, <0.05, respectively). A statistically significant correlation was found between the IL-8 mRNA expression and the colonic inflammation in UC patients (r = 0.44, p < 0.05) but not for SDF1-α expression in UC patients. CONCLUSIONS: IL-8 but not SDF1-α mRNA expression was associated with inflammation in UC. This suggests that IL-8 may play a more important role in inflammatory bowel disease than does SDF1-α. (C) 2000 by Am. Coll. of Gastroenterology.

AB - OBJECTIVES: Interleukin-8 (IL-8) as an α-chemokine recruits and activates neutrophils, which are abundant in the intestinal lesions of ulcerative colitis (UC) and Crohn's disease (CD). Stromal cell-derived factor 1 (SDF1-α) is a new chemokine that is chemotactic to neutrophils. The aims of this study were to assess the relative expression of SDF1-α and IL-8 mRNA in different colonic regions and patients with inflammatory bowel disease with varied degrees of inflammation in the colon. METHODS: Colon biopsy samples were obtained from 19 patients with UC, 12 with CD, and 5 with irritable bowel syndrome (IBS) who underwent colonoscopy. Levels of IL-8 and SDF1-α mRNA expression were measured semiquantitatively by reverse-transcription and polymerase chain reaction amplification. The cytokine mRNA levels were corrected for glyceraldelyde-3-phosphate dehydrogenase mRNA expression. RESULTS: IL-8 mRNA expression was significantly correlated with SDF1-α expression in normal biopsies from IBS patients (r = 0.58, p < 0.01). There was no significant difference in cytokine mRNA expression (IL-8 or SDF1-α) across different regions of the colon or rectum in uninflamed normal biopsies. The IL-8 mRNA expression ratios in UC (mean ± SD, 1.03 ± 0.52) and CD (0.90 ± 0.38) patients were significantly higher than in IBS (0.52 ± 0.17) (p <0.01, p < 0.05, respectively). The SDF1-α mRNA expression ratio in UC (0.30 ± 0.52) was higher than in both CD (0.21 ± 0.10) and IBS patients (0.22 ± 0.11) (p < 0.01, <0.05, respectively). A statistically significant correlation was found between the IL-8 mRNA expression and the colonic inflammation in UC patients (r = 0.44, p < 0.05) but not for SDF1-α expression in UC patients. CONCLUSIONS: IL-8 but not SDF1-α mRNA expression was associated with inflammation in UC. This suggests that IL-8 may play a more important role in inflammatory bowel disease than does SDF1-α. (C) 2000 by Am. Coll. of Gastroenterology.

UR - http://www.scopus.com/inward/record.url?scp=0033761299&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033761299&partnerID=8YFLogxK

U2 - 10.1016/S0002-9270(00)02082-7

DO - 10.1016/S0002-9270(00)02082-7

M3 - Article

C2 - 11095335

AN - SCOPUS:0033761299

VL - 95

SP - 3157

EP - 3164

JO - American Journal of Gastroenterology

JF - American Journal of Gastroenterology

SN - 0002-9270

IS - 11

ER -