TY - JOUR
T1 - Interleukin-8 and SDF1-α mRNA expression in colonic biopsies from patients with inflammatory bowel disease
AU - Katsuta, Takeshi
AU - Lim, Christina
AU - Shimoda, Katsuhiro
AU - Shibuta, Kenji
AU - Mitra, Prasenjit
AU - Banner, Barbara F.
AU - Mori, Masaki
AU - Barnard, Graham F.
PY - 2000/11/20
Y1 - 2000/11/20
N2 - OBJECTIVES: Interleukin-8 (IL-8) as an α-chemokine recruits and activates neutrophils, which are abundant in the intestinal lesions of ulcerative colitis (UC) and Crohn's disease (CD). Stromal cell-derived factor 1 (SDF1-α) is a new chemokine that is chemotactic to neutrophils. The aims of this study were to assess the relative expression of SDF1-α and IL-8 mRNA in different colonic regions and patients with inflammatory bowel disease with varied degrees of inflammation in the colon. METHODS: Colon biopsy samples were obtained from 19 patients with UC, 12 with CD, and 5 with irritable bowel syndrome (IBS) who underwent colonoscopy. Levels of IL-8 and SDF1-α mRNA expression were measured semiquantitatively by reverse-transcription and polymerase chain reaction amplification. The cytokine mRNA levels were corrected for glyceraldelyde-3-phosphate dehydrogenase mRNA expression. RESULTS: IL-8 mRNA expression was significantly correlated with SDF1-α expression in normal biopsies from IBS patients (r = 0.58, p < 0.01). There was no significant difference in cytokine mRNA expression (IL-8 or SDF1-α) across different regions of the colon or rectum in uninflamed normal biopsies. The IL-8 mRNA expression ratios in UC (mean ± SD, 1.03 ± 0.52) and CD (0.90 ± 0.38) patients were significantly higher than in IBS (0.52 ± 0.17) (p <0.01, p < 0.05, respectively). The SDF1-α mRNA expression ratio in UC (0.30 ± 0.52) was higher than in both CD (0.21 ± 0.10) and IBS patients (0.22 ± 0.11) (p < 0.01, <0.05, respectively). A statistically significant correlation was found between the IL-8 mRNA expression and the colonic inflammation in UC patients (r = 0.44, p < 0.05) but not for SDF1-α expression in UC patients. CONCLUSIONS: IL-8 but not SDF1-α mRNA expression was associated with inflammation in UC. This suggests that IL-8 may play a more important role in inflammatory bowel disease than does SDF1-α. (C) 2000 by Am. Coll. of Gastroenterology.
AB - OBJECTIVES: Interleukin-8 (IL-8) as an α-chemokine recruits and activates neutrophils, which are abundant in the intestinal lesions of ulcerative colitis (UC) and Crohn's disease (CD). Stromal cell-derived factor 1 (SDF1-α) is a new chemokine that is chemotactic to neutrophils. The aims of this study were to assess the relative expression of SDF1-α and IL-8 mRNA in different colonic regions and patients with inflammatory bowel disease with varied degrees of inflammation in the colon. METHODS: Colon biopsy samples were obtained from 19 patients with UC, 12 with CD, and 5 with irritable bowel syndrome (IBS) who underwent colonoscopy. Levels of IL-8 and SDF1-α mRNA expression were measured semiquantitatively by reverse-transcription and polymerase chain reaction amplification. The cytokine mRNA levels were corrected for glyceraldelyde-3-phosphate dehydrogenase mRNA expression. RESULTS: IL-8 mRNA expression was significantly correlated with SDF1-α expression in normal biopsies from IBS patients (r = 0.58, p < 0.01). There was no significant difference in cytokine mRNA expression (IL-8 or SDF1-α) across different regions of the colon or rectum in uninflamed normal biopsies. The IL-8 mRNA expression ratios in UC (mean ± SD, 1.03 ± 0.52) and CD (0.90 ± 0.38) patients were significantly higher than in IBS (0.52 ± 0.17) (p <0.01, p < 0.05, respectively). The SDF1-α mRNA expression ratio in UC (0.30 ± 0.52) was higher than in both CD (0.21 ± 0.10) and IBS patients (0.22 ± 0.11) (p < 0.01, <0.05, respectively). A statistically significant correlation was found between the IL-8 mRNA expression and the colonic inflammation in UC patients (r = 0.44, p < 0.05) but not for SDF1-α expression in UC patients. CONCLUSIONS: IL-8 but not SDF1-α mRNA expression was associated with inflammation in UC. This suggests that IL-8 may play a more important role in inflammatory bowel disease than does SDF1-α. (C) 2000 by Am. Coll. of Gastroenterology.
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U2 - 10.1016/S0002-9270(00)02082-7
DO - 10.1016/S0002-9270(00)02082-7
M3 - Article
C2 - 11095335
AN - SCOPUS:0033761299
VL - 95
SP - 3157
EP - 3164
JO - American Journal of Gastroenterology
JF - American Journal of Gastroenterology
SN - 0002-9270
IS - 11
ER -