Interleukin-8 is a major neutrophil chemotactic factor derived from cultured human gingival fibroblasts stimulated with interleukin-1β or tumor necrosis factor alpha

S. Takashiba, M. Takigawa, K. Takahashi, F. Myokai, F. Nishimura, T. Chihara, H. Kurihara, Y. Nomura, Y. Murayama

Research output: Contribution to journalArticle

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Abstract

Inflammatory mediators produced by cells in the gingiva have been implicated in the initiation and progression of periodontal disease, a common infectious disease. In this study, we examined the biological activity of neutrophil chemotactic factors and the kinetics of expression of interleukin- 8 (IL-8) mRNA derived from normal gingival fibroblasts in response to inflammatory mediators in an in vitro model. Gingival fibroblasts stimulated by either recombinant human interleukin-1β or recombinant human tumor necrosis factor alpha produced neutrophil chemotactic factors after 4 h, whereas expression of cell-derived IL-8 mRNA was detected within 1 h after stimulation. Furthermore, in a neutralization assay, rabbit anti-recombinant human IL-8 antiserum inhibited neutrophil chemotactic activity to basal levels. These results provide evidence that gingival fibroblasts synthesize potent chemotactic factors such as IL-8 in the presence of the inflammatory mediators interleukin-1β and tumor necrosis factor alpha. The activity of these factors may contribute to neutrophil-mediated processes in the pathogenesis of periodontal disease.

Original languageEnglish
Pages (from-to)5253-5258
Number of pages6
JournalInfection and Immunity
Volume60
Issue number12
Publication statusPublished - Jan 1 1992
Externally publishedYes

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Interleukin-8
Interleukin-1
Tumor Necrosis Factor-alpha
Fibroblasts
Periodontal Diseases
Neutrophils
Messenger RNA
Chemotactic Factors
Gingiva
Communicable Diseases
Immune Sera
Rabbits

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Interleukin-8 is a major neutrophil chemotactic factor derived from cultured human gingival fibroblasts stimulated with interleukin-1β or tumor necrosis factor alpha. / Takashiba, S.; Takigawa, M.; Takahashi, K.; Myokai, F.; Nishimura, F.; Chihara, T.; Kurihara, H.; Nomura, Y.; Murayama, Y.

In: Infection and Immunity, Vol. 60, No. 12, 01.01.1992, p. 5253-5258.

Research output: Contribution to journalArticle

Takashiba, S, Takigawa, M, Takahashi, K, Myokai, F, Nishimura, F, Chihara, T, Kurihara, H, Nomura, Y & Murayama, Y 1992, 'Interleukin-8 is a major neutrophil chemotactic factor derived from cultured human gingival fibroblasts stimulated with interleukin-1β or tumor necrosis factor alpha', Infection and Immunity, vol. 60, no. 12, pp. 5253-5258.
Takashiba, S. ; Takigawa, M. ; Takahashi, K. ; Myokai, F. ; Nishimura, F. ; Chihara, T. ; Kurihara, H. ; Nomura, Y. ; Murayama, Y. / Interleukin-8 is a major neutrophil chemotactic factor derived from cultured human gingival fibroblasts stimulated with interleukin-1β or tumor necrosis factor alpha. In: Infection and Immunity. 1992 ; Vol. 60, No. 12. pp. 5253-5258.
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AU - Myokai, F.

AU - Nishimura, F.

AU - Chihara, T.

AU - Kurihara, H.

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AB - Inflammatory mediators produced by cells in the gingiva have been implicated in the initiation and progression of periodontal disease, a common infectious disease. In this study, we examined the biological activity of neutrophil chemotactic factors and the kinetics of expression of interleukin- 8 (IL-8) mRNA derived from normal gingival fibroblasts in response to inflammatory mediators in an in vitro model. Gingival fibroblasts stimulated by either recombinant human interleukin-1β or recombinant human tumor necrosis factor alpha produced neutrophil chemotactic factors after 4 h, whereas expression of cell-derived IL-8 mRNA was detected within 1 h after stimulation. Furthermore, in a neutralization assay, rabbit anti-recombinant human IL-8 antiserum inhibited neutrophil chemotactic activity to basal levels. These results provide evidence that gingival fibroblasts synthesize potent chemotactic factors such as IL-8 in the presence of the inflammatory mediators interleukin-1β and tumor necrosis factor alpha. The activity of these factors may contribute to neutrophil-mediated processes in the pathogenesis of periodontal disease.

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