Interleukin‐10 selectively inhibits osteoclastogenesis by inhibiting differentiation of osteoclast progenitors into preosteoclast‐like cells in rat bone marrow culture system

Li Xin Xu, Toshio Kukita, Akiko Kukita, Takeshi Otsuka, Yoshiyuki Niho, Tadahiko Iijima

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

Recombinant human interleukin‐10 (hIL‐10) inhibited the formation of osteoclast‐like multinucleated cells in rat whole bone marrow cultures. The effect of hIL‐10 on the process of osteoclast formation was further examined, since the process of osteoclast formation includes the proliferation and the differentiation of osteoclast progenitors into mononuclear preosteoclasts and the fusion of preosteoclasts into multinucleated osteoclasts. In the nonadherent bone marrow cell culture system, which was free of stromal cells and formed preosteoclast‐like cells, hIL‐10 significantly inhibited the formation of preosteoclast‐like cells even at a very low concentration (0.5 U/ml). The strong inhibition appeared even after treatment with hIL‐10 for only the first 24 h of the culture. However, hIL‐10 did not affect the fusion process of preosteoclast‐like cells to form osteoclast‐like multinucleated cells in the rat coculture system of preosteoclast‐like cells with primary osteo‐blasts. Furthermore, hIL‐10 completely inhibited the colony formation induced by granulocyte macrophage colony‐stimulating factor (GM‐CSF). These findings suggest that the inhibition of osteoclastogenesis by hIL‐10 started at the early stage of the differentiation of osteoclast progenitors to preosteoclasts. © 1995 Wiley‐Liss Inc.

Original languageEnglish
Pages (from-to)624-629
Number of pages6
JournalJournal of cellular physiology
Volume165
Issue number3
DOIs
Publication statusPublished - Dec 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Interleukin‐10 selectively inhibits osteoclastogenesis by inhibiting differentiation of osteoclast progenitors into preosteoclast‐like cells in rat bone marrow culture system'. Together they form a unique fingerprint.

Cite this