Interruption of a human nuclear sequence homologous to mitochondrial DNA by a member of the KpnI 1.8 kb family

Hisayuki Nomiyama, Teruhisa Tsuzuki, Shoji Wakasugi, Makoto Fukuda, Kazunori Shimada

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We reported that several DNA sequences homologous to mitochondrial DNA (mtDNA) are present in the human nuclear genome (Tsuzuki et al. (1983) Gene 25, 223-229). Detailed Southern blot analyses revealed that one of such sequences is interrupted by a repetitive sequence about 1.8 kb long, and that the insert is one member of the dispersed repeated DNA sequences of the KpnI 1.8 kb family. Nucleotide sequence analysis showed that the KpnI 1.8 kb DNA is flanked with imperfect 15-base pair (bp) direct repeats of mtDNA. This KpnI 1.8 kb DNA has an A-rich sequence at its 3'-end, and has a considerable homology with one of the published cDNA sequences homologous to one of the human KpnI families and also to one of the African green monkey KpnI families, KpnI-LS1. These structural features suggest that the KpnI 1.8 kb DNA is a movable element and is inserted within the mtDNA-like sequence by an RNA-mediated process.

Original languageEnglish
Pages (from-to)5225-5234
Number of pages10
JournalNucleic acids research
Volume12
Issue number13
DOIs
Publication statusPublished - Jul 11 1984

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Sequence Homology
Mitochondrial DNA
Nucleic Acid Repetitive Sequences
DNA
Cercopithecus aethiops
Human Genome
Southern Blotting
Base Pairing
Sequence Analysis
Complementary DNA
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Interruption of a human nuclear sequence homologous to mitochondrial DNA by a member of the KpnI 1.8 kb family. / Nomiyama, Hisayuki; Tsuzuki, Teruhisa; Wakasugi, Shoji; Fukuda, Makoto; Shimada, Kazunori.

In: Nucleic acids research, Vol. 12, No. 13, 11.07.1984, p. 5225-5234.

Research output: Contribution to journalArticle

Nomiyama, Hisayuki ; Tsuzuki, Teruhisa ; Wakasugi, Shoji ; Fukuda, Makoto ; Shimada, Kazunori. / Interruption of a human nuclear sequence homologous to mitochondrial DNA by a member of the KpnI 1.8 kb family. In: Nucleic acids research. 1984 ; Vol. 12, No. 13. pp. 5225-5234.
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