Interspecies linkage analysis of mo, a Bombyx mori locus associated with mosaicism and gynandromorphism

T. Fujii, H. Abe, K. Yamamoto, S. Katsuma, T. Shimada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The mo (hereditary mosaic) mutation is one of the most famous and interesting mutations of the silkworm, Bombyx mori. Females homozygous for mo generate mosaic and gynandromorphic offspring due to non-elimination of polar bodies and subsequent double fertilization events, irrespective of the geno type of the mated males. Althoughmo was first reported in 1927, the locus has not been mapped to linkage groups, as the mutation is unstable and appears to be sensitive to genetic background. In this study, linkage analysis of mo was performed using PCR-based markers on single nucleotide polymorphism linkage maps. Bombyx mandarina was used as the mating partner for the B. morimo strain, as it is easier to identify polymorphic markers between B. mori and B. mandarina than within B. mori strains. Surprisingly, we identified two homozygous linkage groups (LGs) in all of the 12 B 1 (first backcross generation) moths that had deposited mosaic eggs. It was revealed that + mo is located on the M chromosome of B. mandarina, which corresponds to two linkage groups of B. mori, LG 14 and 27. Based on further linkage analysis using B. mori as a mating partner, mo was mapped to LG 14. Additionally, we found that mo activity could be modified by a gene(s) on LG 17.

Original languageEnglish
Pages (from-to)1323-1329
Number of pages7
JournalGenetica
Volume139
Issue number10
DOIs
Publication statusPublished - Oct 1 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

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