Intestinal type alkaline phosphatase hyperphosphatasemia associated with liver cirrhosis

Shuichi Saheki, Masaaki Ochi, Takahiko Horiuchi, Yoshikatsu Sakagishi, Yoko Fujimori-Arai, Iwao Koyama, Tsugikazu Komoda, Nozomu Takeuchi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Hyperphosphatasemia due to increased intestinal type serum alkaline phosphatase was noted in a 48-year-old male who had asymptomatic liver cirrhosis. The alkaline phosphatase activity in the serum was 828 U/l (our reference range in adults: 57-194 U/l), 94% of which was of the intestinal type as measured by an immunoprecipitation method. The intestinal component of alkaline phosphatase was separated into two major and some minor components using electrophoresis and isoelectrofocusing. One of the major components had similar mobility to that of a standard intestinal enzyme purified from adult intestine. The components were heat-labile and neuraminidase-resistant. Serial lectin affinity chromatography, however, indicated that sugar chain compositions of the alkaline phosphatase were different from those of the standard tissue intestinal enzyme. These results and further enzymological studies suggest that the patient's serum alkaline phophatase basically consisted of several intestine-like isoforms.

Original languageEnglish
Pages (from-to)63-73
Number of pages11
JournalClinica Chimica Acta
Volume210
Issue number1-2
DOIs
Publication statusPublished - Sep 15 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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