Intracellular accumulation of free calcium in mouse tumor cells exposed to anticancer drugs

S. Kohnoe, Y. Maehara, Y. Emi, I. Takahashi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Alterations in concentrations of intracellular free calcium ([Ca2+]i) were examined in mouse sarcoma S-180 cells exposed to cisplatin, mitomycin C and adriamycin, as related to cell viability. The [Ca2+i was measured using the fluorescent Ca2+ indicator, fura-2. During the first 24 hr of incubation of the cells in vitro, in the presence of the drugs, the [Ca2+]i began to increase at a time when the cell viability was reduced to 50% of the controls, and finally exceeded 1 μM with reduction to 10%. The increase in [Ca2+]i was reciprocal to the loss of cell viability. Changes in Ca2+ concentrations in the medium did not alter the results. These findings suggest that in the presence of anticancer drugs there is an accumulation of free Ca2+ with a release of this ion from internal stores and that cell death follows disruption in the homeostasis of intracellular Ca2+.

Original languageEnglish
Pages (from-to)2203-2207
Number of pages5
JournalAnticancer research
Volume12
Issue number6 B
Publication statusPublished - Dec 1 1992

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Intracellular accumulation of free calcium in mouse tumor cells exposed to anticancer drugs'. Together they form a unique fingerprint.

  • Cite this

    Kohnoe, S., Maehara, Y., Emi, Y., & Takahashi, I. (1992). Intracellular accumulation of free calcium in mouse tumor cells exposed to anticancer drugs. Anticancer research, 12(6 B), 2203-2207.