Intracellular accumulation of toxic turn amyloid-β is associated with endoplasmic reticulum stress in Alzheimer's disease

Naoko Soejima, Yasumasa Ohyagi, Norimichi Nakamura, Eri Himeno, Kyoko M. Iinuma, Nobutaka Sakae, Ryo Yamasaki, Takeshi Tabira, Kazuma Murakami, Kazuhiro Irie, Noriaki Kinoshita, Frank M. LaFerla, Yutaka Kiyohara, Toru Iwaki, Jun ichi Kira

Research output: Contribution to journalArticle

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Abstract

Amyloid-β3 protein (Aβ3) accumulates in the neurons of Alzheimer's disease (AD) patients at an early stage of the disease. Recently, we found that Aβ3 with a toxic turn at positions 22 and 23 accumulates in neurons in AD brain. Here, we studied the accumulation of Aβ3, toxic turn Aβ3 and high-molecular-weight Aβ3 oligomers in presenilin 1 (PS1) gene-transfected SH-SY5Y cells as well as in the brains of 3xTg-AD mice and AD patients. Immunostaining revealed that accumulation of toxic turn Aβ3 was promoted in G384A- and I143T-mutant PS1-transfected cells and further enhanced by co-transfection of cells with the Aβ3-precursor protein (Aβ3PP) gene. In contrast, accumulation of high-molecular-weight Aβ3 oligomers was promoted in mutant PS1 cells but attenuated by co-transfection of cells with the Aβ3PP gene. Toxic turn Aβ3 was detected in the neurons of 3xTg-AD mice aged 2 months, when the mice were cognitively unimpaired. In contrast, high-molecular-weight Aβ3 oligomers were detected in the neurons of 7-month-old mice, when memory dysfunction is apparent. Furthermore, immunostaining and western blotting for Rab4, Rab6 and GRP78 revealed increased levels of these proteins in mutant PS1 cells and their accumulation in the neurons of 3xTg-AD mice. Remarkably, GRP78 immunoreactivity was increased at 2 months of age. Double-label immunostaining of AD brain revealed an apparent association between toxic turn Aβ3 and GRP78, an endoplasmic reticulum (ER) stress marker. Intraneuronal accumulation of toxic turn Aβ3 may be associated with ER stress in the brains of AD model mice and AD patients at an early stage.

Original languageEnglish
Pages (from-to)11-20
Number of pages10
JournalCurrent Alzheimer Research
Volume10
Issue number1
Publication statusPublished - Nov 29 2013

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Amyloidogenic Proteins
Endoplasmic Reticulum Stress
Poisons
Amyloid
Alzheimer Disease
Presenilin-1
Neurons
Molecular Weight
Transfection
Brain
Protein Precursors
Amyloid beta-Protein Precursor
Brain Diseases
Mutant Proteins
Proteins
Western Blotting

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Intracellular accumulation of toxic turn amyloid-β is associated with endoplasmic reticulum stress in Alzheimer's disease. / Soejima, Naoko; Ohyagi, Yasumasa; Nakamura, Norimichi; Himeno, Eri; Iinuma, Kyoko M.; Sakae, Nobutaka; Yamasaki, Ryo; Tabira, Takeshi; Murakami, Kazuma; Irie, Kazuhiro; Kinoshita, Noriaki; LaFerla, Frank M.; Kiyohara, Yutaka; Iwaki, Toru; Kira, Jun ichi.

In: Current Alzheimer Research, Vol. 10, No. 1, 29.11.2013, p. 11-20.

Research output: Contribution to journalArticle

Soejima, N, Ohyagi, Y, Nakamura, N, Himeno, E, Iinuma, KM, Sakae, N, Yamasaki, R, Tabira, T, Murakami, K, Irie, K, Kinoshita, N, LaFerla, FM, Kiyohara, Y, Iwaki, T & Kira, JI 2013, 'Intracellular accumulation of toxic turn amyloid-β is associated with endoplasmic reticulum stress in Alzheimer's disease', Current Alzheimer Research, vol. 10, no. 1, pp. 11-20.
Soejima, Naoko ; Ohyagi, Yasumasa ; Nakamura, Norimichi ; Himeno, Eri ; Iinuma, Kyoko M. ; Sakae, Nobutaka ; Yamasaki, Ryo ; Tabira, Takeshi ; Murakami, Kazuma ; Irie, Kazuhiro ; Kinoshita, Noriaki ; LaFerla, Frank M. ; Kiyohara, Yutaka ; Iwaki, Toru ; Kira, Jun ichi. / Intracellular accumulation of toxic turn amyloid-β is associated with endoplasmic reticulum stress in Alzheimer's disease. In: Current Alzheimer Research. 2013 ; Vol. 10, No. 1. pp. 11-20.
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