Intracellular acidification is required for full activation of the sweet taste receptor by miraculin

Keisuke Sanematsu, Masayuki Kitagawa, Ryusuke Yoshida, Satoru Nirasawa, Noriatsu Shigemura, Yuzo Ninomiya

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Acidification of the glycoprotein, miraculin (MCL), induces sweet taste in humans, but not in mice. The sweet taste induced by MCL is more intense when acidification occurs with weak acids as opposed to strong acids. MCL interacts with the human sweet receptor subunit hTAS1R2, but the mechanisms by which the acidification of MCL activates the sweet taste receptor remain largely unexplored. The work reported here speaks directly to this activation by utilizing a sweet receptor TAS1R2 + TAS1R3 assay. In accordance with previous data, MCL-applied cells displayed a pH dependence with citric acid (weak acid) being right shifted to that with hydrochloric acid (strong acid). When histidine residues in both the intracellular and extracellular region of hTAS1R2 were exchanged for alanine, taste-modifying effect of MCL was reduced or abolished. Stronger intracellular acidification of HEK293 cells was induced by citric acid than by HCl and taste-modifying effect of MCL was proportional to intracellular pH regardless of types of acids. These results suggest that intracellular acidity is required for full activation of the sweet taste receptor by MCL.

Original languageEnglish
Article number22807
JournalScientific reports
Volume6
DOIs
Publication statusPublished - Mar 10 2016

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Acids
Citric Acid
Hydrochloric Acid
HEK293 Cells
Histidine
Alanine
Glycoproteins

All Science Journal Classification (ASJC) codes

  • General

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Intracellular acidification is required for full activation of the sweet taste receptor by miraculin. / Sanematsu, Keisuke; Kitagawa, Masayuki; Yoshida, Ryusuke; Nirasawa, Satoru; Shigemura, Noriatsu; Ninomiya, Yuzo.

In: Scientific reports, Vol. 6, 22807, 10.03.2016.

Research output: Contribution to journalArticle

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