Intracellular Ca2+ Responses in Cultured Endothelial Cells to Mechanical Stimulation by Laser Tweezers

Noriyuki Kataoka, Ken Hashimoto, Susumu Kudo, Ryuhei Yamaguchi, Katsuhiko Tsujioka, Fumihiko Kajiya

Research output: Contribution to journalArticle

Abstract

It is well known that endothelial cells (ECs) respond to the fluid imposed shear I stress and change their shapes and functions. We have focused on the importance of cytoplasmic micro-mechanical strain in mechano-sensing mechanism of ECs. To this end, the cytosolic Ca2+ responses of ECs to the mechanical stimulus by laser tweezers that can apply the micro-mechanical force to nano/micro-organisms without any physical contact, were investigated. When the laser spot focused on the nucleus of EC was slightly moved, the cytosolic Ca2+ increased immediately in the same EC, whereas there was no Ca2+ increase without laser spot movement. In the absence of extracellular Ca2+ in the medium or the blockade of stretch activated ion channels, there was also an increase of Ca2+ in stimulated ECs. Therefore, the increased Ca2+ in stimulated ECs is considered to be derived from intracellular Ca2+ store. The heterogeneous Ca2+ propagation from the stimulated EC to surrounding ECs was also observed. Two types of Ca2+ wave propagation were observed, the fast one that the velocity was more than 20μm/sec, and the slow one that the velocity was less than 1μm/sec. The micro-stress induced by the micro-movement of the nucleus can be a trigger of the cytosolic Ca2+ increase and the cytoplasmic micro-mechanical strain may play an important role in mechano-sensing mechanism of ECs.

Original languageEnglish
Pages (from-to)116-123
Number of pages8
JournalJournal of Biomechanical Science and Engineering
Volume3
Issue number2
DOIs
Publication statusPublished - Jan 1 2008
Externally publishedYes

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Endothelial cells
Lasers
Wave propagation
Fluids

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering

Cite this

Intracellular Ca2+ Responses in Cultured Endothelial Cells to Mechanical Stimulation by Laser Tweezers. / Kataoka, Noriyuki; Hashimoto, Ken; Kudo, Susumu; Yamaguchi, Ryuhei; Tsujioka, Katsuhiko; Kajiya, Fumihiko.

In: Journal of Biomechanical Science and Engineering, Vol. 3, No. 2, 01.01.2008, p. 116-123.

Research output: Contribution to journalArticle

Kataoka, Noriyuki ; Hashimoto, Ken ; Kudo, Susumu ; Yamaguchi, Ryuhei ; Tsujioka, Katsuhiko ; Kajiya, Fumihiko. / Intracellular Ca2+ Responses in Cultured Endothelial Cells to Mechanical Stimulation by Laser Tweezers. In: Journal of Biomechanical Science and Engineering. 2008 ; Vol. 3, No. 2. pp. 116-123.
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