Intracellular Ca2+ transient phase II can be assessed by half-logistic function model in isolated aequorin-injected mouse left ventricular papillary muscle

Ju Mizuno, Mikiya Otsuji, Hideko Arita, Kazuo Hanaoka, Takeshi Yokoyama

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Abstract

Background: Myocardial contraction and relaxation are regulated by increases and decreases in intracellular cytoplasmic calcium (Ca2+) concentration ([Ca2+]i). In previous studies, we found that a half-logistic (h-L) function, which represents a half-curve of a symmetrical sigmoid logistic function with a boundary at the inflection point, curve-fits the first half of the ascending phase (CaTI) and the second half of the descending phase of the [Ca2+]i transient curve (CaTIV) better than a mono-exponential (m-E) function. In the present study, we investigated the potential application of an h-L function to the analysis of the second half of the ascending phase of the [Ca2+]i transient curve (CaTII). Methods: The [Ca2+]i transient was measured using the Ca2+-sensitive photoprotein aequorin, which was microinjected into 15 isolated left ventricular (LV) papillary muscles of mice. The observed CaTII data during the time duration from the point corresponding to the maximum of the first-order time derivative of Ca2+ concentration (dCa/dtmax) to the point corresponding to the peak Ca2+ concentration was curve-fitted by the least-squares method using the h-L and m-E function equations. Results: The mean correlation coefficient (r) values of the h-L and m-E curve-fits for CaTII were 0.9996 and 0.9984, respectively. The Z transformation of h-L r was larger than that of m-E r (p < 0.0001). H-L residual mean square (RMS) was smaller than m-E RMS (p < 0.001). Conclusions: The h-L function tracks the magnitudes and time courses of CaTII more accurately than the m-E function in isolated aequorin-injected mouse LV papillary muscle. Compared with the m-E time constant, the h-L time constant of CaTII is a more reliable index for evaluating the time duration of the change in the increase in [Ca2+]i during the combination of the middle part of the contraction process and the early part of the relaxation process. CaTII can be assessed by the h-L function model in cardiac muscles. The h-L approach may provide amore useful model for studying each process in myocardial Ca2+ handling.

Original languageEnglish
Pages (from-to)328-338
Number of pages11
JournalActa Cardiologica Sinica
Volume29
Issue number4
Publication statusPublished - Jul 1 2013

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Aequorin
Papillary Muscles
Logistic Models
Luminescent Proteins
Myocardial Contraction
Sigmoid Colon
Least-Squares Analysis
Myocardium
Calcium

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Intracellular Ca2+ transient phase II can be assessed by half-logistic function model in isolated aequorin-injected mouse left ventricular papillary muscle. / Mizuno, Ju; Otsuji, Mikiya; Arita, Hideko; Hanaoka, Kazuo; Yokoyama, Takeshi.

In: Acta Cardiologica Sinica, Vol. 29, No. 4, 01.07.2013, p. 328-338.

Research output: Contribution to journalArticle

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T1 - Intracellular Ca2+ transient phase II can be assessed by half-logistic function model in isolated aequorin-injected mouse left ventricular papillary muscle

AU - Mizuno, Ju

AU - Otsuji, Mikiya

AU - Arita, Hideko

AU - Hanaoka, Kazuo

AU - Yokoyama, Takeshi

PY - 2013/7/1

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N2 - Background: Myocardial contraction and relaxation are regulated by increases and decreases in intracellular cytoplasmic calcium (Ca2+) concentration ([Ca2+]i). In previous studies, we found that a half-logistic (h-L) function, which represents a half-curve of a symmetrical sigmoid logistic function with a boundary at the inflection point, curve-fits the first half of the ascending phase (CaTI) and the second half of the descending phase of the [Ca2+]i transient curve (CaTIV) better than a mono-exponential (m-E) function. In the present study, we investigated the potential application of an h-L function to the analysis of the second half of the ascending phase of the [Ca2+]i transient curve (CaTII). Methods: The [Ca2+]i transient was measured using the Ca2+-sensitive photoprotein aequorin, which was microinjected into 15 isolated left ventricular (LV) papillary muscles of mice. The observed CaTII data during the time duration from the point corresponding to the maximum of the first-order time derivative of Ca2+ concentration (dCa/dtmax) to the point corresponding to the peak Ca2+ concentration was curve-fitted by the least-squares method using the h-L and m-E function equations. Results: The mean correlation coefficient (r) values of the h-L and m-E curve-fits for CaTII were 0.9996 and 0.9984, respectively. The Z transformation of h-L r was larger than that of m-E r (p < 0.0001). H-L residual mean square (RMS) was smaller than m-E RMS (p < 0.001). Conclusions: The h-L function tracks the magnitudes and time courses of CaTII more accurately than the m-E function in isolated aequorin-injected mouse LV papillary muscle. Compared with the m-E time constant, the h-L time constant of CaTII is a more reliable index for evaluating the time duration of the change in the increase in [Ca2+]i during the combination of the middle part of the contraction process and the early part of the relaxation process. CaTII can be assessed by the h-L function model in cardiac muscles. The h-L approach may provide amore useful model for studying each process in myocardial Ca2+ handling.

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