Intracellular distribution of pancreatic tumor antigen purified from bhp-induced adenocarcinomas in syrian hamsters

Masaharu Matsuzaki, Tsuneo Natori, Kazuhiro Mizumoto, Shunji Kitazawa, Masahiro Tsutsumi, Yoichi Konishi

Research output: Contribution to journalArticle

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Abstract

Pancreatic tumor antigen (PTA) was purified to apparent homogeneity from hamster pancreatic adenocarcinomas by a rapid and simple procedure using column chromatography on Mono P and Superose 6. Amino acid analysis indicated essential similarities between hamster PTA and human pancreatic cancer-associated antigen (PCAA, PCAAc), although differences in the contents of several amino acid residues between the two proteins did suggest species variation. Investigation of the binding pattern of antibody raised against PTA by peroxidase-labeled antibody immunocytochemistry revealed that, whereas PTA is absent or only faintly expressed in normal epithelium, it is very strongly positive in transplantable hamster pancreatic adenocarcinomas induced by nitrosamines. PTA was found to be distributed over the entire surface of neoplastic cells and it was concluded that the presence of PTA on the basolateral surface of malignant pancreatic epithelial cells, where it. might have access to the blood circulation, could be one explanation for the observed elevated concentrations of PTA in the serum. PTA is thus a potential marker for hamster neoplastic pancreatobiliary duct-type cells.

Original languageEnglish
Pages (from-to)429-435
Number of pages7
JournalPancreas
Volume4
Issue number4
DOIs
Publication statusPublished - Jan 1 1989
Externally publishedYes

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Mesocricetus
Neoplasm Antigens
Adenocarcinoma
Cricetinae
Amino Acids
Nitrosamines
Antibodies
Blood Circulation
Pancreatic Neoplasms
Peroxidase
Chromatography
Epithelium
Epithelial Cells
Immunohistochemistry
Antigens
Serum

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Cite this

Intracellular distribution of pancreatic tumor antigen purified from bhp-induced adenocarcinomas in syrian hamsters. / Matsuzaki, Masaharu; Natori, Tsuneo; Mizumoto, Kazuhiro; Kitazawa, Shunji; Tsutsumi, Masahiro; Konishi, Yoichi.

In: Pancreas, Vol. 4, No. 4, 01.01.1989, p. 429-435.

Research output: Contribution to journalArticle

Matsuzaki, Masaharu ; Natori, Tsuneo ; Mizumoto, Kazuhiro ; Kitazawa, Shunji ; Tsutsumi, Masahiro ; Konishi, Yoichi. / Intracellular distribution of pancreatic tumor antigen purified from bhp-induced adenocarcinomas in syrian hamsters. In: Pancreas. 1989 ; Vol. 4, No. 4. pp. 429-435.
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