Abstract
Cellular distribution of the antimutagenic MTH1 protein in the liver, kidney, and testis of Fischer rat was evaluated using the immunohistochemical staining with anti-MTH1 polyclonal antibody. The present investigation revealed a non-uniform distribution of MTH1 among cells and among the cytoplasmic, nuclear, and membranal structures of cells within a given tissue. A particularly strong expression of MTH1 was observed for the first time in the perinuclear acrosomic bodies of spermatocytes and in the acrosomic vesicles of sperm heads. Treatment of rats with a single sc dose of 20 μmol Cd(II)/kg body wt. produced histopathologic changes in these organs accompanied by redistribution of the cellular MTH1 protein between the cytoplasm and nuclei. The acute phase of Cd(II) toxicity, that in the liver and especially in the testes (but not in kidneys) led to cell necrosis, was accompanied by a characteristic decrease in the abundance of MTH1-expressing nuclei. Chronic toxicity without necrosis, persisting in the kidney over the entire 14-day study, as well as the survival and proliferation of cells, observed in the liver and testis after the necrotizing phase, were signified by increased number of nuclei expressing MTH1. Thus, unlike previous biochemical studies, immunohistochemistry managed to reveal alterations in the patterns of inter- and intracellular distribution of MTH1, associated apparently with the conditional changes in the dynamics of synthesis of nucleic acids, assisted by this protein.
| Original language | English |
|---|---|
| Article number | 1 |
| Pages (from-to) | 325-335 |
| Number of pages | 11 |
| Journal | Experimental and Toxicologic Pathology |
| Volume | 53 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jan 1 2001 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Toxicology
- Cell Biology
Cite this
Intracellular distribution of the antimutagenic enzyme MTH1 in the liver, kidney and testis of F344 rats and its modulation by cadmium. / Kasprzak, K. S.; Nakabeppu, Yusaku; Kakuma, T.; Sakai, Yasunari; Tsuruya, Kazuhiko; Sekiguchi, M.; Ward, J. M.; Diwan, B. A.; Nagashima, K.; Kasprzak, B. H.
In: Experimental and Toxicologic Pathology, Vol. 53, No. 5, 1, 01.01.2001, p. 325-335.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intracellular distribution of the antimutagenic enzyme MTH1 in the liver, kidney and testis of F344 rats and its modulation by cadmium
AU - Kasprzak, K. S.
AU - Nakabeppu, Yusaku
AU - Kakuma, T.
AU - Sakai, Yasunari
AU - Tsuruya, Kazuhiko
AU - Sekiguchi, M.
AU - Ward, J. M.
AU - Diwan, B. A.
AU - Nagashima, K.
AU - Kasprzak, B. H.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Cellular distribution of the antimutagenic MTH1 protein in the liver, kidney, and testis of Fischer rat was evaluated using the immunohistochemical staining with anti-MTH1 polyclonal antibody. The present investigation revealed a non-uniform distribution of MTH1 among cells and among the cytoplasmic, nuclear, and membranal structures of cells within a given tissue. A particularly strong expression of MTH1 was observed for the first time in the perinuclear acrosomic bodies of spermatocytes and in the acrosomic vesicles of sperm heads. Treatment of rats with a single sc dose of 20 μmol Cd(II)/kg body wt. produced histopathologic changes in these organs accompanied by redistribution of the cellular MTH1 protein between the cytoplasm and nuclei. The acute phase of Cd(II) toxicity, that in the liver and especially in the testes (but not in kidneys) led to cell necrosis, was accompanied by a characteristic decrease in the abundance of MTH1-expressing nuclei. Chronic toxicity without necrosis, persisting in the kidney over the entire 14-day study, as well as the survival and proliferation of cells, observed in the liver and testis after the necrotizing phase, were signified by increased number of nuclei expressing MTH1. Thus, unlike previous biochemical studies, immunohistochemistry managed to reveal alterations in the patterns of inter- and intracellular distribution of MTH1, associated apparently with the conditional changes in the dynamics of synthesis of nucleic acids, assisted by this protein.
AB - Cellular distribution of the antimutagenic MTH1 protein in the liver, kidney, and testis of Fischer rat was evaluated using the immunohistochemical staining with anti-MTH1 polyclonal antibody. The present investigation revealed a non-uniform distribution of MTH1 among cells and among the cytoplasmic, nuclear, and membranal structures of cells within a given tissue. A particularly strong expression of MTH1 was observed for the first time in the perinuclear acrosomic bodies of spermatocytes and in the acrosomic vesicles of sperm heads. Treatment of rats with a single sc dose of 20 μmol Cd(II)/kg body wt. produced histopathologic changes in these organs accompanied by redistribution of the cellular MTH1 protein between the cytoplasm and nuclei. The acute phase of Cd(II) toxicity, that in the liver and especially in the testes (but not in kidneys) led to cell necrosis, was accompanied by a characteristic decrease in the abundance of MTH1-expressing nuclei. Chronic toxicity without necrosis, persisting in the kidney over the entire 14-day study, as well as the survival and proliferation of cells, observed in the liver and testis after the necrotizing phase, were signified by increased number of nuclei expressing MTH1. Thus, unlike previous biochemical studies, immunohistochemistry managed to reveal alterations in the patterns of inter- and intracellular distribution of MTH1, associated apparently with the conditional changes in the dynamics of synthesis of nucleic acids, assisted by this protein.
UR - http://www.scopus.com/inward/record.url?scp=0035158162&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035158162&partnerID=8YFLogxK
U2 - 10.1078/0940-2993-00201
DO - 10.1078/0940-2993-00201
M3 - Article
C2 - 11817101
AN - SCOPUS:0035158162
VL - 53
SP - 325
EP - 335
JO - Experimental and Toxicologic Pathology
JF - Experimental and Toxicologic Pathology
SN - 0940-2993
IS - 5
M1 - 1
ER -