Intracellular electrolyte levels and transport of secretory granules in exocrine gland cells

Sadao Sasaki, Ikuko Nakagaki, Seiki Hori, Hisao Kondo, Bungo Sakaguchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We demonstrate the intracellular transport of secretory granules of a silk protein, fibroin, from the Golgi region to the apical cytoplasm with special reference to microtubule organization, electrolyte concentrations and the acidic intragranular pH of normal and mutant posterior silk gland cells, using the techniques of electrophysiological microelectrode and microprobe analysis and of light and electron microscopic autoradiography. The silk gland cells of a recessive mutant making only flimsy cocoons were defective in the microtubule systems, did not stain with an anti-tubulin antibody in immunofluorescent microscopy, and accumulated intracellular granules in the apical and basal cytoplasm. The increase in intracellular calcium concentration and levels of chloride secretion were also reduced in the mutant cells. A carboxylic ionophore, monensin, which collapsed the granular H+ gradient, induced the transport of chloride and an increase in the intracellular calcium concentration, while it blocked the intracellular transport of granules from the Golgi region to the apical cytoplasm in normal cells. Thus, we conclude that the H+ gradient across the membrane of secretory granules is responsible for the intracellular transport of the secretory granules along the microtubule systems in silk gland cells, while Ca2+ is thought to be required for the exocytosis of the granules.

Original languageEnglish
Pages (from-to)1273-1281
Number of pages9
JournalJournal of Experimental Biology
Volume201
Issue number9
Publication statusPublished - May 1998
Externally publishedYes

Fingerprint

Exocrine Glands
exocrine glands
secretory granules
Secretory Vesicles
cytoplasm
Silk
electrolyte
Electrolytes
electrolytes
silk glands
Microtubules
microtubules
granules
calcium
Cytoplasm
chloride
cocoon
mutants
cells
Chlorides

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Intracellular electrolyte levels and transport of secretory granules in exocrine gland cells. / Sasaki, Sadao; Nakagaki, Ikuko; Hori, Seiki; Kondo, Hisao; Sakaguchi, Bungo.

In: Journal of Experimental Biology, Vol. 201, No. 9, 05.1998, p. 1273-1281.

Research output: Contribution to journalArticle

Sasaki, S, Nakagaki, I, Hori, S, Kondo, H & Sakaguchi, B 1998, 'Intracellular electrolyte levels and transport of secretory granules in exocrine gland cells', Journal of Experimental Biology, vol. 201, no. 9, pp. 1273-1281.
Sasaki, Sadao ; Nakagaki, Ikuko ; Hori, Seiki ; Kondo, Hisao ; Sakaguchi, Bungo. / Intracellular electrolyte levels and transport of secretory granules in exocrine gland cells. In: Journal of Experimental Biology. 1998 ; Vol. 201, No. 9. pp. 1273-1281.
@article{5bd35e1fbc214462b8cddafc56a16f7f,
title = "Intracellular electrolyte levels and transport of secretory granules in exocrine gland cells",
abstract = "We demonstrate the intracellular transport of secretory granules of a silk protein, fibroin, from the Golgi region to the apical cytoplasm with special reference to microtubule organization, electrolyte concentrations and the acidic intragranular pH of normal and mutant posterior silk gland cells, using the techniques of electrophysiological microelectrode and microprobe analysis and of light and electron microscopic autoradiography. The silk gland cells of a recessive mutant making only flimsy cocoons were defective in the microtubule systems, did not stain with an anti-tubulin antibody in immunofluorescent microscopy, and accumulated intracellular granules in the apical and basal cytoplasm. The increase in intracellular calcium concentration and levels of chloride secretion were also reduced in the mutant cells. A carboxylic ionophore, monensin, which collapsed the granular H+ gradient, induced the transport of chloride and an increase in the intracellular calcium concentration, while it blocked the intracellular transport of granules from the Golgi region to the apical cytoplasm in normal cells. Thus, we conclude that the H+ gradient across the membrane of secretory granules is responsible for the intracellular transport of the secretory granules along the microtubule systems in silk gland cells, while Ca2+ is thought to be required for the exocytosis of the granules.",
author = "Sadao Sasaki and Ikuko Nakagaki and Seiki Hori and Hisao Kondo and Bungo Sakaguchi",
year = "1998",
month = "5",
language = "English",
volume = "201",
pages = "1273--1281",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists Ltd",
number = "9",

}

TY - JOUR

T1 - Intracellular electrolyte levels and transport of secretory granules in exocrine gland cells

AU - Sasaki, Sadao

AU - Nakagaki, Ikuko

AU - Hori, Seiki

AU - Kondo, Hisao

AU - Sakaguchi, Bungo

PY - 1998/5

Y1 - 1998/5

N2 - We demonstrate the intracellular transport of secretory granules of a silk protein, fibroin, from the Golgi region to the apical cytoplasm with special reference to microtubule organization, electrolyte concentrations and the acidic intragranular pH of normal and mutant posterior silk gland cells, using the techniques of electrophysiological microelectrode and microprobe analysis and of light and electron microscopic autoradiography. The silk gland cells of a recessive mutant making only flimsy cocoons were defective in the microtubule systems, did not stain with an anti-tubulin antibody in immunofluorescent microscopy, and accumulated intracellular granules in the apical and basal cytoplasm. The increase in intracellular calcium concentration and levels of chloride secretion were also reduced in the mutant cells. A carboxylic ionophore, monensin, which collapsed the granular H+ gradient, induced the transport of chloride and an increase in the intracellular calcium concentration, while it blocked the intracellular transport of granules from the Golgi region to the apical cytoplasm in normal cells. Thus, we conclude that the H+ gradient across the membrane of secretory granules is responsible for the intracellular transport of the secretory granules along the microtubule systems in silk gland cells, while Ca2+ is thought to be required for the exocytosis of the granules.

AB - We demonstrate the intracellular transport of secretory granules of a silk protein, fibroin, from the Golgi region to the apical cytoplasm with special reference to microtubule organization, electrolyte concentrations and the acidic intragranular pH of normal and mutant posterior silk gland cells, using the techniques of electrophysiological microelectrode and microprobe analysis and of light and electron microscopic autoradiography. The silk gland cells of a recessive mutant making only flimsy cocoons were defective in the microtubule systems, did not stain with an anti-tubulin antibody in immunofluorescent microscopy, and accumulated intracellular granules in the apical and basal cytoplasm. The increase in intracellular calcium concentration and levels of chloride secretion were also reduced in the mutant cells. A carboxylic ionophore, monensin, which collapsed the granular H+ gradient, induced the transport of chloride and an increase in the intracellular calcium concentration, while it blocked the intracellular transport of granules from the Golgi region to the apical cytoplasm in normal cells. Thus, we conclude that the H+ gradient across the membrane of secretory granules is responsible for the intracellular transport of the secretory granules along the microtubule systems in silk gland cells, while Ca2+ is thought to be required for the exocytosis of the granules.

UR - http://www.scopus.com/inward/record.url?scp=0031804335&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031804335&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0031804335

VL - 201

SP - 1273

EP - 1281

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

IS - 9

ER -