Intracellular hypoxia of tumor tissue estimated by noninvasive electron paramagnetic resonance oximetry technique using paramagnetic probes

Atsuko Matsumoto, Ken Ichiro Matsumoto, Shingo Matsumoto, Fuminori Hyodo, Anastasia L. Sowers, Janusz W. Koscielniak, Nallathamby Devasahayam, Sankaran Subramanian, James B. Mitchell, Murali C. Krishna

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Electron paramagnetic resonance (EPR) oximetry at 700 MHz operating frequency employing a surface coil resonator is used to assess tissue partial pressure of oxygen (pO2) using paramagnetic media whose linewidth and decay constant are related to oxygen concentration. Differences in extracellular and intracellular pO2 in squamous cell carcinoma (SCC) tumor tissue were tested using several types of water-soluble paramagnetic media, which localize extracellularly or permeate through the cell membrane. The nitroxide carboxy-PROXYL (CxP) can only be distributed in blood plasma and extracellular fluids whereas the nitroxides carbamoyl-PROXYL (CmP) and TEMPOL (TPL) can permeate cell membranes and localize intracellularly. EPR signal decay constant and the linewidth of the intravenously administered nitroxides in SCC tumor tissues implanted in mouse thigh and the contralateral normal muscle of healthy mice breathing gases with different pO2 were compared. The pO2 in the blood can depend on the oxygen content in the breathing gas while tissue pO2 was not directly influenced by pO2 in the breathing gas. The decay constants of CmP and TPL in tumor tissue were significantly larger than in the normal muscles, and lower linewidths of CmP and TPL in tumor tissue was observed. The SCC tumor showed intracellular hypoxia even though the extracellular pO2 is similar to normal tissue in the peripheral region.

    Original languageEnglish
    Pages (from-to)142-145
    Number of pages4
    JournalBiological and Pharmaceutical Bulletin
    Volume34
    Issue number1
    DOIs
    Publication statusPublished - Jan 28 2011

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    Oximetry
    Electron Spin Resonance Spectroscopy
    Squamous Cell Carcinoma
    Respiration
    Gases
    Neoplasms
    Oxygen
    Cell Membrane
    Muscles
    Partial Pressure
    Extracellular Fluid
    Thigh
    Tumor Hypoxia
    Water

    All Science Journal Classification (ASJC) codes

    • Pharmacology
    • Pharmaceutical Science

    Cite this

    Matsumoto, A., Matsumoto, K. I., Matsumoto, S., Hyodo, F., Sowers, A. L., Koscielniak, J. W., ... Krishna, M. C. (2011). Intracellular hypoxia of tumor tissue estimated by noninvasive electron paramagnetic resonance oximetry technique using paramagnetic probes. Biological and Pharmaceutical Bulletin, 34(1), 142-145. https://doi.org/10.1248/bpb.34.142

    Intracellular hypoxia of tumor tissue estimated by noninvasive electron paramagnetic resonance oximetry technique using paramagnetic probes. / Matsumoto, Atsuko; Matsumoto, Ken Ichiro; Matsumoto, Shingo; Hyodo, Fuminori; Sowers, Anastasia L.; Koscielniak, Janusz W.; Devasahayam, Nallathamby; Subramanian, Sankaran; Mitchell, James B.; Krishna, Murali C.

    In: Biological and Pharmaceutical Bulletin, Vol. 34, No. 1, 28.01.2011, p. 142-145.

    Research output: Contribution to journalArticle

    Matsumoto, A, Matsumoto, KI, Matsumoto, S, Hyodo, F, Sowers, AL, Koscielniak, JW, Devasahayam, N, Subramanian, S, Mitchell, JB & Krishna, MC 2011, 'Intracellular hypoxia of tumor tissue estimated by noninvasive electron paramagnetic resonance oximetry technique using paramagnetic probes', Biological and Pharmaceutical Bulletin, vol. 34, no. 1, pp. 142-145. https://doi.org/10.1248/bpb.34.142
    Matsumoto, Atsuko ; Matsumoto, Ken Ichiro ; Matsumoto, Shingo ; Hyodo, Fuminori ; Sowers, Anastasia L. ; Koscielniak, Janusz W. ; Devasahayam, Nallathamby ; Subramanian, Sankaran ; Mitchell, James B. ; Krishna, Murali C. / Intracellular hypoxia of tumor tissue estimated by noninvasive electron paramagnetic resonance oximetry technique using paramagnetic probes. In: Biological and Pharmaceutical Bulletin. 2011 ; Vol. 34, No. 1. pp. 142-145.
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