Intracellular signal-responsive artificial gene regulation for novel gene delivery

Yoshiki Katayama, Kenji Fujii, Etsuko Ito, Shigeki Sakakihara, Tatsuhiko Sonoda, Masaharu Murata, Mizuo Maeda

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

We describe two types of artificial gene-regulation systems responding to cyclic AMP-dependent protein kinase (PKA) or caspase-3. These molecular systems use newly synthesized cationic polymers, PAK and PAC. The PAK polymer includes substrate oligopeptide for PKA, ARRASLG, as receptor of PKA signal, while the PAC polymer possesses oligopeptide that is comprised of a substrate sequence of caspase-3, DEVD, and a cationic oligolysine, KKKKKK. These polymers formed stable complexes with DNA to totally suppress the gene expression. However, PKA or caspase-3 signal disintegrates the PAK-DNA or the PAC-DNA complex, respectively. This liberates the DNA and activated the gene expression. These systems are the first concept of an intracellular signal-responsive gene-regulation system using artificial polymer. We expect that these systems can be applied to the novel highly cell specific gene delivery strategy that is involved in our previously proposed new drug delivery concept, the drug delivery system based on responses to cellular signals.

Original languageEnglish
Pages (from-to)905-909
Number of pages5
JournalBiomacromolecules
Volume3
Issue number5
DOIs
Publication statusPublished - Sep 1 2002

Fingerprint

Synthetic Genes
Gene expression
Polymers
Genes
DNA
Caspase 3
Protein Kinases
Proteins
Oligopeptides
Gene Expression
Substrates
Drug Delivery Systems
Cyclic AMP-Dependent Protein Kinases
Drug delivery
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Katayama, Y., Fujii, K., Ito, E., Sakakihara, S., Sonoda, T., Murata, M., & Maeda, M. (2002). Intracellular signal-responsive artificial gene regulation for novel gene delivery. Biomacromolecules, 3(5), 905-909. https://doi.org/10.1021/bm025532h

Intracellular signal-responsive artificial gene regulation for novel gene delivery. / Katayama, Yoshiki; Fujii, Kenji; Ito, Etsuko; Sakakihara, Shigeki; Sonoda, Tatsuhiko; Murata, Masaharu; Maeda, Mizuo.

In: Biomacromolecules, Vol. 3, No. 5, 01.09.2002, p. 905-909.

Research output: Contribution to journalArticle

Katayama, Y, Fujii, K, Ito, E, Sakakihara, S, Sonoda, T, Murata, M & Maeda, M 2002, 'Intracellular signal-responsive artificial gene regulation for novel gene delivery', Biomacromolecules, vol. 3, no. 5, pp. 905-909. https://doi.org/10.1021/bm025532h
Katayama, Yoshiki ; Fujii, Kenji ; Ito, Etsuko ; Sakakihara, Shigeki ; Sonoda, Tatsuhiko ; Murata, Masaharu ; Maeda, Mizuo. / Intracellular signal-responsive artificial gene regulation for novel gene delivery. In: Biomacromolecules. 2002 ; Vol. 3, No. 5. pp. 905-909.
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