Intracellular signal-responsive gene carrier for cell-specific gene expression

Kenji Kawamura, Jun Oishi, Jeong Hun Kang, Kota Kodama, Tatsuhiko Sonoda, Masaharu Murata, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We designed a peptide - polymer conjugate (CPCCtat) as a novel gene carrier that could control gene expression responding to the intracellular caspase-3 signal. This carrier consists of an uncharged main polymer chain and a cationic peptide side chain, which includes the substrate sequence of caspase-3 and the protein transduction domain sequence of HIV-1 Tat. In the present study, CPCCtat formed a tight complex with DNA through an electrostatic interaction, and in this state the gene expression was totally suppressed. In contrast, the complex disintegrated in the presence of caspase-3 due to cleavage of the cationic portion from CPCCtat. This event led to an activation of gene expression. Our results also indicate that the complex can be delivered into living cells due to the cell-permeable peptide side chain of CPCCtat. This intracellular signal-responsive system with CPCCtat will be useful for the cell-specific gene expression system.

Original languageEnglish
Pages (from-to)908-913
Number of pages6
JournalBiomacromolecules
Volume6
Issue number2
DOIs
Publication statusPublished - Mar 1 2005

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Gene expression
Genes
Caspase 3
Peptides
Polymers
Signal systems
Coulomb interactions
DNA
Chemical activation
Cells
Proteins
Substrates

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Kawamura, K., Oishi, J., Kang, J. H., Kodama, K., Sonoda, T., Murata, M., ... Katayama, Y. (2005). Intracellular signal-responsive gene carrier for cell-specific gene expression. Biomacromolecules, 6(2), 908-913. https://doi.org/10.1021/bm0493887

Intracellular signal-responsive gene carrier for cell-specific gene expression. / Kawamura, Kenji; Oishi, Jun; Kang, Jeong Hun; Kodama, Kota; Sonoda, Tatsuhiko; Murata, Masaharu; Niidome, Takuro; Katayama, Yoshiki.

In: Biomacromolecules, Vol. 6, No. 2, 01.03.2005, p. 908-913.

Research output: Contribution to journalArticle

Kawamura, K, Oishi, J, Kang, JH, Kodama, K, Sonoda, T, Murata, M, Niidome, T & Katayama, Y 2005, 'Intracellular signal-responsive gene carrier for cell-specific gene expression', Biomacromolecules, vol. 6, no. 2, pp. 908-913. https://doi.org/10.1021/bm0493887
Kawamura K, Oishi J, Kang JH, Kodama K, Sonoda T, Murata M et al. Intracellular signal-responsive gene carrier for cell-specific gene expression. Biomacromolecules. 2005 Mar 1;6(2):908-913. https://doi.org/10.1021/bm0493887
Kawamura, Kenji ; Oishi, Jun ; Kang, Jeong Hun ; Kodama, Kota ; Sonoda, Tatsuhiko ; Murata, Masaharu ; Niidome, Takuro ; Katayama, Yoshiki. / Intracellular signal-responsive gene carrier for cell-specific gene expression. In: Biomacromolecules. 2005 ; Vol. 6, No. 2. pp. 908-913.
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