Intracellular transport and processing of lysosomal cathepsin B

Yukio Nishimura, Keitaro Kato

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Intracellular transport and processing of lysosomal cathepsin B was investigated in the subcellular fractions of rat liver by pulse-labeling experiments with [35S]methionine in vivo. A newly synthesized procathepsin B with a molecular weight of 39 kDa firstly appeared in the rough microsomal fraction at 10 min postinjection of label. This procathepsin B moved from the microsomal fractions to the Golgi subfractions at 30 min postinjection, and then a processed mature enzyme appeared in the lysosomal fraction at 60 min. These results suggest that the propeptide-processing of procathepsin B takes place in lysosomes in the course of intracellular transport from endoplasmic reticulum through Golgi complex to lysosomes.

Original languageEnglish
Pages (from-to)254-259
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume148
Issue number1
DOIs
Publication statusPublished - Oct 14 1987

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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