Intracellular transport and processing of lysosomal cathepsin B

Yukio Nishimura, Keitaro Kato

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Intracellular transport and processing of lysosomal cathepsin B was investigated in the subcellular fractions of rat liver by pulse-labeling experiments with [35S]methionine in vivo. A newly synthesized procathepsin B with a molecular weight of 39 kDa firstly appeared in the rough microsomal fraction at 10 min postinjection of label. This procathepsin B moved from the microsomal fractions to the Golgi subfractions at 30 min postinjection, and then a processed mature enzyme appeared in the lysosomal fraction at 60 min. These results suggest that the propeptide-processing of procathepsin B takes place in lysosomes in the course of intracellular transport from endoplasmic reticulum through Golgi complex to lysosomes.

Original languageEnglish
Pages (from-to)254-259
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume148
Issue number1
DOIs
Publication statusPublished - Oct 14 1987

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Cathepsin B
Lysosomes
Processing
Subcellular Fractions
Golgi Apparatus
Endoplasmic Reticulum
Methionine
Liver
Labeling
Rats
Labels
Molecular Weight
Molecular weight
Enzymes
procathepsin B
Experiments

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Intracellular transport and processing of lysosomal cathepsin B. / Nishimura, Yukio; Kato, Keitaro.

In: Biochemical and Biophysical Research Communications, Vol. 148, No. 1, 14.10.1987, p. 254-259.

Research output: Contribution to journalArticle

Nishimura, Yukio ; Kato, Keitaro. / Intracellular transport and processing of lysosomal cathepsin B. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 148, No. 1. pp. 254-259.
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