TY - JOUR
T1 - Intracellular transport and processing of lysosomal cathepsin B
AU - Nishimura, Yukio
AU - Kato, Keitaro
N1 - Funding Information:
This work was supported in part Research from the Ministry Japan and a Grant from the
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1987/10/14
Y1 - 1987/10/14
N2 - Intracellular transport and processing of lysosomal cathepsin B was investigated in the subcellular fractions of rat liver by pulse-labeling experiments with [35S]methionine in vivo. A newly synthesized procathepsin B with a molecular weight of 39 kDa firstly appeared in the rough microsomal fraction at 10 min postinjection of label. This procathepsin B moved from the microsomal fractions to the Golgi subfractions at 30 min postinjection, and then a processed mature enzyme appeared in the lysosomal fraction at 60 min. These results suggest that the propeptide-processing of procathepsin B takes place in lysosomes in the course of intracellular transport from endoplasmic reticulum through Golgi complex to lysosomes.
AB - Intracellular transport and processing of lysosomal cathepsin B was investigated in the subcellular fractions of rat liver by pulse-labeling experiments with [35S]methionine in vivo. A newly synthesized procathepsin B with a molecular weight of 39 kDa firstly appeared in the rough microsomal fraction at 10 min postinjection of label. This procathepsin B moved from the microsomal fractions to the Golgi subfractions at 30 min postinjection, and then a processed mature enzyme appeared in the lysosomal fraction at 60 min. These results suggest that the propeptide-processing of procathepsin B takes place in lysosomes in the course of intracellular transport from endoplasmic reticulum through Golgi complex to lysosomes.
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U2 - 10.1016/0006-291X(87)91103-X
DO - 10.1016/0006-291X(87)91103-X
M3 - Article
C2 - 3675577
AN - SCOPUS:0023653461
VL - 148
SP - 254
EP - 259
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -