Intracellular transport of the measles virus ribonucleoprotein complex is mediated by Rab11A-positive recycling endosomes and drives virus release from the apical membrane of polarized epithelial cells

Yuichiro Nakatsu, Xuemin Ma, Fumio Seki, Tadaki Suzuki, Masaharu Iwasaki, Yusuke Yanagi, Katsuhiro Komase, Makoto Takedaa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Many viruses use the host trafficking system at a variety of their replication steps. Measles virus (MV) possesses a nonsegmented negative-strand RNA genome that encodes three components of the ribonucleoprotein (RNP) complex (N, P, and L), two surface glycoproteins, a matrix protein, and two nonstructural proteins. A subset of immune cells and polarized epithelial cells are in vivo targets of MV, and MV is selectively released from the apical membrane of polarized epithelial cells. However, the molecular mechanisms for the apical release of MV remain largely unknown. In the present study,the localization and trafficking mechanisms of the RNP complex of MV were analyzed in detail using recombinant MVs expressing fluorescent protein-tagged L proteins. Live cell imaging analyses demonstrated that the MV RNP complex was transported in a manner dependent on the microtubule network and together with Rab11A-containing recycling endosomes. The RNP complex was accumulated at the apical membrane and the apical recycling compartment. The accumulation and shedding of infectious virions were severely impaired by expression of a dominant negative form of Rab11A. On the other hand, recycling endosome-mediated RNP transport was totally dispensable for virus production in nonpolarized cells. These data providethe first demonstration of the regulated intracellular trafficking events of the MV RNP complex that define the directional viral release from polarized epithelial cells.

Original languageEnglish
Pages (from-to)4683-4693
Number of pages11
JournalJournal of virology
Volume87
Issue number8
DOIs
Publication statusPublished - Apr 1 2013

Fingerprint

Measles virus
Virus Release
ribonucleoproteins
endosomes
Ribonucleoproteins
Endosomes
recycling
epithelial cells
Epithelial Cells
viruses
Membranes
Proteins
Viruses
proteins
Membrane Glycoproteins
Recycling
cells
virion
Microtubules
Virion

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Intracellular transport of the measles virus ribonucleoprotein complex is mediated by Rab11A-positive recycling endosomes and drives virus release from the apical membrane of polarized epithelial cells. / Nakatsu, Yuichiro; Ma, Xuemin; Seki, Fumio; Suzuki, Tadaki; Iwasaki, Masaharu; Yanagi, Yusuke; Komase, Katsuhiro; Takedaa, Makoto.

In: Journal of virology, Vol. 87, No. 8, 01.04.2013, p. 4683-4693.

Research output: Contribution to journalArticle

Nakatsu, Yuichiro ; Ma, Xuemin ; Seki, Fumio ; Suzuki, Tadaki ; Iwasaki, Masaharu ; Yanagi, Yusuke ; Komase, Katsuhiro ; Takedaa, Makoto. / Intracellular transport of the measles virus ribonucleoprotein complex is mediated by Rab11A-positive recycling endosomes and drives virus release from the apical membrane of polarized epithelial cells. In: Journal of virology. 2013 ; Vol. 87, No. 8. pp. 4683-4693.
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