TY - JOUR
T1 - Intracerebral microinjections of Δ9-tetrahydrocannabinol
T2 - Search for the impairment of spatial memory in the eight-arm radial maze in rats
AU - Egashira, Nobuaki
AU - Mishima, Kenichi
AU - Iwasaki, Katsunori
AU - Fujiwara, Michihiro
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid for Scientific Research (No. 12771472) from the Japanese Ministry of Education, Culture, Sports, Science and Technology and a Research Grants (10A-3 and 13A-3) for Nervous and Mental Disorders from the Japanese Ministry of Health, Labour and Welfare. The authors are grateful to Professor Y. Shoyama, Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, for kindly supplying natural Δ 9 -tetrahydrocannabinol.
PY - 2002/10/18
Y1 - 2002/10/18
N2 - The purpose of this study was to identify brain sites that contribute to the Δ9-tetrahydrocannabinol (Δ9-THC)-induced impairment of spatial memory in rats. Rats were tested in the eight-arm radial maze after microinjections of Δ9-THC into one of 14 different brain regions. The bilateral microinjection of Δ9-THC (20 μg/side) impaired spatial memory when injected into the dorsal hippocampus (DH), ventral hippocampus (VH) or dorsomedial thalamus nucleus (DMT). However, rats treated with Δ9-THC into DMT produced perseverative behavior which has not been observed by systemic administration of Δ9-THC. On the other hand, spatial memory was unaffected by microinjections of Δ9-THC into the other 11 areas examined: frontal (FC) and frontoparietal (FPC) cortex, central (ACE) and basolateral (ABL) amygdaloid nucleus, medial caudate putamen (CPM), lateral hypothalamus (LH), mammillary body (MB), basal forebrain (BF), medial septal nucleus (SEP) and dorsal (DR) and median (MR) raphe nucleus. These results suggest that DH and VH may be important brain sites for the Δ9-THC-induced impairment of spatial memory.
AB - The purpose of this study was to identify brain sites that contribute to the Δ9-tetrahydrocannabinol (Δ9-THC)-induced impairment of spatial memory in rats. Rats were tested in the eight-arm radial maze after microinjections of Δ9-THC into one of 14 different brain regions. The bilateral microinjection of Δ9-THC (20 μg/side) impaired spatial memory when injected into the dorsal hippocampus (DH), ventral hippocampus (VH) or dorsomedial thalamus nucleus (DMT). However, rats treated with Δ9-THC into DMT produced perseverative behavior which has not been observed by systemic administration of Δ9-THC. On the other hand, spatial memory was unaffected by microinjections of Δ9-THC into the other 11 areas examined: frontal (FC) and frontoparietal (FPC) cortex, central (ACE) and basolateral (ABL) amygdaloid nucleus, medial caudate putamen (CPM), lateral hypothalamus (LH), mammillary body (MB), basal forebrain (BF), medial septal nucleus (SEP) and dorsal (DR) and median (MR) raphe nucleus. These results suggest that DH and VH may be important brain sites for the Δ9-THC-induced impairment of spatial memory.
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U2 - 10.1016/S0006-8993(02)03247-X
DO - 10.1016/S0006-8993(02)03247-X
M3 - Article
C2 - 12376185
AN - SCOPUS:0037131069
VL - 952
SP - 239
EP - 245
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 2
ER -