TY - JOUR
T1 - Intracerebroventricular injection of ghrelin and growth hormone releasing factor inhibits food intake in neonatal chicks
AU - Furuse, Mitsuhiro
AU - Tachibana, Tetsuya
AU - Ohgushi, Atsushi
AU - Ando, Ryuichi
AU - Yoshimatsu, Takao
AU - Denbow, D. Michael
N1 - Funding Information:
This study was supported by a grant-in-aid for scientific research from the Ministry of Education, Science and Culture, Japan and Uehara Memorial Foundation.
PY - 2001/3/30
Y1 - 2001/3/30
N2 - Growth hormone releasing factor (GRF) is known to stimulate feeding of rats. Ghrelin, a novel growth hormone (GH)-releasing acylated peptide, was recently isolated from rat stomach. It also stimulates the release of GH from the anterior pituitary through the GH secretagogue receptor (GHS-R) and feeding in the rat. We have investigated the effects of ghrelin and GRF on food intake of the neonatal chick. In Experiment 1, 0, 1.25, 2.5 and 5 μg of ghrelin were administered intracerebroventricularly (i.c.v.) to ad libitum fed birds. In Experiment 2, the effect of (i.c.v.) injection of 0, 1.25, 2.5 and 5 μg of GRF was investigated. Both peptides strongly inhibited food intake of the chick during the 2-h post-injection period. In the third experiment, 0, 0.5, 1 and 2 μg of ghrelin was injected i.c.v. in chicks previously deprived of food for 3 h. Food intake was again inhibited by ghrelin in a dose-dependent manner. These results suggest that the mechanisms for feeding of the neonatal chick through GH release are different from mammals.
AB - Growth hormone releasing factor (GRF) is known to stimulate feeding of rats. Ghrelin, a novel growth hormone (GH)-releasing acylated peptide, was recently isolated from rat stomach. It also stimulates the release of GH from the anterior pituitary through the GH secretagogue receptor (GHS-R) and feeding in the rat. We have investigated the effects of ghrelin and GRF on food intake of the neonatal chick. In Experiment 1, 0, 1.25, 2.5 and 5 μg of ghrelin were administered intracerebroventricularly (i.c.v.) to ad libitum fed birds. In Experiment 2, the effect of (i.c.v.) injection of 0, 1.25, 2.5 and 5 μg of GRF was investigated. Both peptides strongly inhibited food intake of the chick during the 2-h post-injection period. In the third experiment, 0, 0.5, 1 and 2 μg of ghrelin was injected i.c.v. in chicks previously deprived of food for 3 h. Food intake was again inhibited by ghrelin in a dose-dependent manner. These results suggest that the mechanisms for feeding of the neonatal chick through GH release are different from mammals.
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U2 - 10.1016/S0304-3940(01)01621-4
DO - 10.1016/S0304-3940(01)01621-4
M3 - Article
C2 - 11248438
AN - SCOPUS:0035970816
VL - 301
SP - 123
EP - 126
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -