Intracerebroventricular injection of L-proline and D-proline induces sedative and hypnotic effects by different mechanisms under an acute stressful condition in chicks

K. Hamasu, K. Shigemi, Y. Tsuneyoshi, H. Yamane, H. Sato, D. M. Denbow, M. Furuse

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The central effects of L-proline, D-proline and trans-4-hydroxy-L-proline were investigated by using the acute stressful model with neonatal chicks in Experiment 1. Sedative and hypnotic effects were induced by all compounds, while plasma corticosterone release under isolation stress was only attenuated by L-proline. To clarify the mechanism by which L-proline and D-proline induce sedative and hypnotic effects, the contribution of the strychnine-sensitive glycine receptor (glycine receptor) and N-methyl-D-aspartate glutamate receptor (NMDA receptor) were further investigated. In Experiments 2-3, the glycine receptor antagonist strychnine was co-injected intracerebroventricular (i.c.v.) with L-proline or D-proline. The suppression of isolation-induced stress behavior by D-proline was attenuated by strychnine. However, the suppression of stress behavior by L-proline was not attenuated. In Experiment 4, the NMDA receptor antagonist (+)-MK-801 was co-injected i.c.v. with L-proline. The suppression of stress behavior by L-proline was attenuated by (+)-MK-801. These results indicate that L-proline and D-proline differentially induce sedative and hypnotic effects through NMDA and glycine receptors, respectively.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalAmino Acids
Volume38
Issue number1
DOIs
Publication statusPublished - Jan 1 2010

Fingerprint

Hypnotics and Sedatives
Proline
Injections
Glycine Receptors
Strychnine
N-Methyl-D-Aspartate Receptors
Dizocilpine Maleate
N-Methylaspartate
Excitatory Amino Acid Antagonists
Experiments
Glutamate Receptors
Corticosterone
Plasmas

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Intracerebroventricular injection of L-proline and D-proline induces sedative and hypnotic effects by different mechanisms under an acute stressful condition in chicks. / Hamasu, K.; Shigemi, K.; Tsuneyoshi, Y.; Yamane, H.; Sato, H.; Denbow, D. M.; Furuse, M.

In: Amino Acids, Vol. 38, No. 1, 01.01.2010, p. 57-64.

Research output: Contribution to journalArticle

@article{3c21794102734814afbe7c63f440ebdd,
title = "Intracerebroventricular injection of L-proline and D-proline induces sedative and hypnotic effects by different mechanisms under an acute stressful condition in chicks",
abstract = "The central effects of L-proline, D-proline and trans-4-hydroxy-L-proline were investigated by using the acute stressful model with neonatal chicks in Experiment 1. Sedative and hypnotic effects were induced by all compounds, while plasma corticosterone release under isolation stress was only attenuated by L-proline. To clarify the mechanism by which L-proline and D-proline induce sedative and hypnotic effects, the contribution of the strychnine-sensitive glycine receptor (glycine receptor) and N-methyl-D-aspartate glutamate receptor (NMDA receptor) were further investigated. In Experiments 2-3, the glycine receptor antagonist strychnine was co-injected intracerebroventricular (i.c.v.) with L-proline or D-proline. The suppression of isolation-induced stress behavior by D-proline was attenuated by strychnine. However, the suppression of stress behavior by L-proline was not attenuated. In Experiment 4, the NMDA receptor antagonist (+)-MK-801 was co-injected i.c.v. with L-proline. The suppression of stress behavior by L-proline was attenuated by (+)-MK-801. These results indicate that L-proline and D-proline differentially induce sedative and hypnotic effects through NMDA and glycine receptors, respectively.",
author = "K. Hamasu and K. Shigemi and Y. Tsuneyoshi and H. Yamane and H. Sato and Denbow, {D. M.} and M. Furuse",
year = "2010",
month = "1",
day = "1",
doi = "10.1007/s00726-008-0204-9",
language = "English",
volume = "38",
pages = "57--64",
journal = "Amino Acids",
issn = "0939-4451",
publisher = "Springer Wien",
number = "1",

}

TY - JOUR

T1 - Intracerebroventricular injection of L-proline and D-proline induces sedative and hypnotic effects by different mechanisms under an acute stressful condition in chicks

AU - Hamasu, K.

AU - Shigemi, K.

AU - Tsuneyoshi, Y.

AU - Yamane, H.

AU - Sato, H.

AU - Denbow, D. M.

AU - Furuse, M.

PY - 2010/1/1

Y1 - 2010/1/1

N2 - The central effects of L-proline, D-proline and trans-4-hydroxy-L-proline were investigated by using the acute stressful model with neonatal chicks in Experiment 1. Sedative and hypnotic effects were induced by all compounds, while plasma corticosterone release under isolation stress was only attenuated by L-proline. To clarify the mechanism by which L-proline and D-proline induce sedative and hypnotic effects, the contribution of the strychnine-sensitive glycine receptor (glycine receptor) and N-methyl-D-aspartate glutamate receptor (NMDA receptor) were further investigated. In Experiments 2-3, the glycine receptor antagonist strychnine was co-injected intracerebroventricular (i.c.v.) with L-proline or D-proline. The suppression of isolation-induced stress behavior by D-proline was attenuated by strychnine. However, the suppression of stress behavior by L-proline was not attenuated. In Experiment 4, the NMDA receptor antagonist (+)-MK-801 was co-injected i.c.v. with L-proline. The suppression of stress behavior by L-proline was attenuated by (+)-MK-801. These results indicate that L-proline and D-proline differentially induce sedative and hypnotic effects through NMDA and glycine receptors, respectively.

AB - The central effects of L-proline, D-proline and trans-4-hydroxy-L-proline were investigated by using the acute stressful model with neonatal chicks in Experiment 1. Sedative and hypnotic effects were induced by all compounds, while plasma corticosterone release under isolation stress was only attenuated by L-proline. To clarify the mechanism by which L-proline and D-proline induce sedative and hypnotic effects, the contribution of the strychnine-sensitive glycine receptor (glycine receptor) and N-methyl-D-aspartate glutamate receptor (NMDA receptor) were further investigated. In Experiments 2-3, the glycine receptor antagonist strychnine was co-injected intracerebroventricular (i.c.v.) with L-proline or D-proline. The suppression of isolation-induced stress behavior by D-proline was attenuated by strychnine. However, the suppression of stress behavior by L-proline was not attenuated. In Experiment 4, the NMDA receptor antagonist (+)-MK-801 was co-injected i.c.v. with L-proline. The suppression of stress behavior by L-proline was attenuated by (+)-MK-801. These results indicate that L-proline and D-proline differentially induce sedative and hypnotic effects through NMDA and glycine receptors, respectively.

UR - http://www.scopus.com/inward/record.url?scp=76449083141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76449083141&partnerID=8YFLogxK

U2 - 10.1007/s00726-008-0204-9

DO - 10.1007/s00726-008-0204-9

M3 - Article

C2 - 19023642

AN - SCOPUS:76449083141

VL - 38

SP - 57

EP - 64

JO - Amino Acids

JF - Amino Acids

SN - 0939-4451

IS - 1

ER -