TY - JOUR
T1 - Intracerebroventricular injection taurine changes free amino acid concentrations in the brain and plasma in chicks
AU - Elhussiny, Mohamed Z.
AU - Tran, Phuong V.
AU - Wang, Ying
AU - Ouchi, Yoshimitsu
AU - Haraguchi, Shogo
AU - Gilbert, Elizabeth R.
AU - Cline, Mark A.
AU - Bungo, Takashi
AU - Furuse, Mitsuhiro
AU - Chowdhury, Vishwajit S.
N1 - Funding Information:
M.Z.E. was funded by a full scholarship from the Ministry of Higher Education of the Arab Republic of Egypt. This work was supported by JSPS KAKENHI Grant Number JP19H03110 to V.S.C and JP21H02344 to T.B.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Brain amino acid metabolism has been reported to regulate body temperature, feeding behavior and stress response. Central injection of taurine induced hypothermic and anorexigenic effects in chicks. However, it is still unknown how the amino acid metabolism is influenced by the central injection of taurine. Therefore, the objective of this study was to investigate the changes in brain and plasma free amino acids following central injection of taurine. Five-day-old male Julia layer chicks (n = 10) were subjected to intracerebroventricular (ICV) injection with saline or taurine (5 µmol/10 µL). Central taurine increased tryptophan concentrations in the diencephalon, and decreased tyrosine in the diencephalon, brainstem, cerebellum, telencephalon and plasma at 30 min post-injection. Taurine was increased in all the brain parts after ICV taurine. Although histidine and cystathionine concentrations were increased in the diencephalon and brainstem, several amino acids such as isoleucine, arginine, methionine, phenylalanine, glutamic acid, asparagine, proline, and alanine were reduced following central injection of taurine. All amino acid concentrations were decreased in the plasma after ICV taurine. In conclusion, central taurine quickly changes free amino acid concentrations in the brain and plasma, which may have a role in thermoregulation, food intake and stress response in chicks.
AB - Brain amino acid metabolism has been reported to regulate body temperature, feeding behavior and stress response. Central injection of taurine induced hypothermic and anorexigenic effects in chicks. However, it is still unknown how the amino acid metabolism is influenced by the central injection of taurine. Therefore, the objective of this study was to investigate the changes in brain and plasma free amino acids following central injection of taurine. Five-day-old male Julia layer chicks (n = 10) were subjected to intracerebroventricular (ICV) injection with saline or taurine (5 µmol/10 µL). Central taurine increased tryptophan concentrations in the diencephalon, and decreased tyrosine in the diencephalon, brainstem, cerebellum, telencephalon and plasma at 30 min post-injection. Taurine was increased in all the brain parts after ICV taurine. Although histidine and cystathionine concentrations were increased in the diencephalon and brainstem, several amino acids such as isoleucine, arginine, methionine, phenylalanine, glutamic acid, asparagine, proline, and alanine were reduced following central injection of taurine. All amino acid concentrations were decreased in the plasma after ICV taurine. In conclusion, central taurine quickly changes free amino acid concentrations in the brain and plasma, which may have a role in thermoregulation, food intake and stress response in chicks.
UR - http://www.scopus.com/inward/record.url?scp=85142752054&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85142752054&partnerID=8YFLogxK
U2 - 10.1007/s00726-022-03216-7
DO - 10.1007/s00726-022-03216-7
M3 - Article
C2 - 36436082
AN - SCOPUS:85142752054
SN - 0939-4451
JO - Amino Acids
JF - Amino Acids
ER -