Intragenic antagonistic roles of protein and circRNA in tumorigenesis

Jlenia Guarnerio, Yang Zhang, Giulia Cheloni, Riccardo Panella, Jesse Mae Katon, Mark Simpson, Akinobu Matsumoto, Antonella Papa, Cristian Loretelli, Andreas Petri, Sakari Kauppinen, Cassandra Garbutt, Gunnlaugur Petur Nielsen, Vikram Deshpande, Mireia Castillo-Martin, Carlos Cordon-Cardo, Spentzos Dimitrios, John G. Clohessy, Mona Batish, Pier Paolo Pandolfi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

circRNAs arise from back splicing events during mRNA processing, and when deregulated can play an active role in cancer. Here we characterize a new circRNA (circPOK) encoded by the Zbtb7a gene (also kown as POKEMON, LRF) in the context of mesenchymal tumor progression. circPOK functions as a non-coding proto-oncogenic RNA independently and antithetically to its linear transcript counterpart, which acts as a tumor suppressor by encoding the Pokemon transcription factor. We find that circPOK regulates pro-proliferative and pro-angiogenic factors by co-activation of the ILF2/3 complex. Importantly, the expression of Pokemon protein and circRNA is aberrantly uncoupled in cancer through differential post-transcriptional regulation. Thus, we identify a novel type of genetic unit, the iRegulon, that yields biochemically distinct RNA products, circular and linear, with diverse and antithetical functions. Our findings further expand the cellular repertoire towards the control of normal biological outputs, while aberrant expression of such components may underlie disease pathogenesis including cancer.

Original languageEnglish
Pages (from-to)628-640
Number of pages13
JournalCell Research
Volume29
Issue number8
DOIs
Publication statusPublished - Aug 1 2019

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Carcinogenesis
Neoplasms
Proteins
Angiogenesis Inducing Agents
Transcription Factors
RNA
Messenger RNA
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Guarnerio, J., Zhang, Y., Cheloni, G., Panella, R., Mae Katon, J., Simpson, M., ... Pandolfi, P. P. (2019). Intragenic antagonistic roles of protein and circRNA in tumorigenesis. Cell Research, 29(8), 628-640. https://doi.org/10.1038/s41422-019-0192-1

Intragenic antagonistic roles of protein and circRNA in tumorigenesis. / Guarnerio, Jlenia; Zhang, Yang; Cheloni, Giulia; Panella, Riccardo; Mae Katon, Jesse; Simpson, Mark; Matsumoto, Akinobu; Papa, Antonella; Loretelli, Cristian; Petri, Andreas; Kauppinen, Sakari; Garbutt, Cassandra; Petur Nielsen, Gunnlaugur; Deshpande, Vikram; Castillo-Martin, Mireia; Cordon-Cardo, Carlos; Dimitrios, Spentzos; Clohessy, John G.; Batish, Mona; Pandolfi, Pier Paolo.

In: Cell Research, Vol. 29, No. 8, 01.08.2019, p. 628-640.

Research output: Contribution to journalArticle

Guarnerio, J, Zhang, Y, Cheloni, G, Panella, R, Mae Katon, J, Simpson, M, Matsumoto, A, Papa, A, Loretelli, C, Petri, A, Kauppinen, S, Garbutt, C, Petur Nielsen, G, Deshpande, V, Castillo-Martin, M, Cordon-Cardo, C, Dimitrios, S, Clohessy, JG, Batish, M & Pandolfi, PP 2019, 'Intragenic antagonistic roles of protein and circRNA in tumorigenesis', Cell Research, vol. 29, no. 8, pp. 628-640. https://doi.org/10.1038/s41422-019-0192-1
Guarnerio J, Zhang Y, Cheloni G, Panella R, Mae Katon J, Simpson M et al. Intragenic antagonistic roles of protein and circRNA in tumorigenesis. Cell Research. 2019 Aug 1;29(8):628-640. https://doi.org/10.1038/s41422-019-0192-1
Guarnerio, Jlenia ; Zhang, Yang ; Cheloni, Giulia ; Panella, Riccardo ; Mae Katon, Jesse ; Simpson, Mark ; Matsumoto, Akinobu ; Papa, Antonella ; Loretelli, Cristian ; Petri, Andreas ; Kauppinen, Sakari ; Garbutt, Cassandra ; Petur Nielsen, Gunnlaugur ; Deshpande, Vikram ; Castillo-Martin, Mireia ; Cordon-Cardo, Carlos ; Dimitrios, Spentzos ; Clohessy, John G. ; Batish, Mona ; Pandolfi, Pier Paolo. / Intragenic antagonistic roles of protein and circRNA in tumorigenesis. In: Cell Research. 2019 ; Vol. 29, No. 8. pp. 628-640.
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AU - Simpson, Mark

AU - Matsumoto, Akinobu

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AU - Castillo-Martin, Mireia

AU - Cordon-Cardo, Carlos

AU - Dimitrios, Spentzos

AU - Clohessy, John G.

AU - Batish, Mona

AU - Pandolfi, Pier Paolo

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