Abstract
The soybean-nodulating Sinorhizobium fredii strain has been reported to possess three copies of rRNA gene operons. In the present study, we investigated the diversity of the 16S-23S rDNA internal transcribed spacer (ITS) regions of S. fredii strains. Based on the sequences of the ITS regions, we divided the sequences of the S. fredii strains into two groups, type A and type B. A dot-matrix analysis indicated that the region flanked by tRNA-Ile and tRNA-Ala is longer in type A than in type B, whereas type B sequences possess longer regions upstream of tRNA-Ile and downstream of tRNA-Ala than those of the type A sequence. Restriction fragment length polymorphism of polymerase chain reaction product (PCR-RFLP) of the ITS region in the cloned plasmids as templates could reconstruct the PCR-RFLP pattern from the total DNA as a template. The results of Southern hybridization using the insert sequence between tRNA-Ile and tRNA-Ala in type A as a probe indicated differences in the copy numbers of the type A ITS regions among the strains tested. These results indicated that S. fredii strains possess the type A and type B sequences of the ITS regions at ratios of 3:0, 2:1, 1:2 or 0:3. These S. fredii strains may be useful biological materials for the study of intraspecific variations.
| Original language | English |
|---|---|
| Pages (from-to) | 627-633 |
| Number of pages | 7 |
| Journal | Soil Science and Plant Nutrition |
| Volume | 55 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Oct 1 2009 |
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All Science Journal Classification (ASJC) codes
- Soil Science
- Plant Science
Cite this
Intragenomic variation in the internal transcribed spacer regions between 16S-23S rRNA genes among the three copies of Sinorhizobium fredii strains. / Saeki, Yuichi; Oguro, Hiroko; Akagi, Isao; Yamakawa, Takeo; Yamamoto, Akihiro.
In: Soil Science and Plant Nutrition, Vol. 55, No. 5, 01.10.2009, p. 627-633.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intragenomic variation in the internal transcribed spacer regions between 16S-23S rRNA genes among the three copies of Sinorhizobium fredii strains
AU - Saeki, Yuichi
AU - Oguro, Hiroko
AU - Akagi, Isao
AU - Yamakawa, Takeo
AU - Yamamoto, Akihiro
PY - 2009/10/1
Y1 - 2009/10/1
N2 - The soybean-nodulating Sinorhizobium fredii strain has been reported to possess three copies of rRNA gene operons. In the present study, we investigated the diversity of the 16S-23S rDNA internal transcribed spacer (ITS) regions of S. fredii strains. Based on the sequences of the ITS regions, we divided the sequences of the S. fredii strains into two groups, type A and type B. A dot-matrix analysis indicated that the region flanked by tRNA-Ile and tRNA-Ala is longer in type A than in type B, whereas type B sequences possess longer regions upstream of tRNA-Ile and downstream of tRNA-Ala than those of the type A sequence. Restriction fragment length polymorphism of polymerase chain reaction product (PCR-RFLP) of the ITS region in the cloned plasmids as templates could reconstruct the PCR-RFLP pattern from the total DNA as a template. The results of Southern hybridization using the insert sequence between tRNA-Ile and tRNA-Ala in type A as a probe indicated differences in the copy numbers of the type A ITS regions among the strains tested. These results indicated that S. fredii strains possess the type A and type B sequences of the ITS regions at ratios of 3:0, 2:1, 1:2 or 0:3. These S. fredii strains may be useful biological materials for the study of intraspecific variations.
AB - The soybean-nodulating Sinorhizobium fredii strain has been reported to possess three copies of rRNA gene operons. In the present study, we investigated the diversity of the 16S-23S rDNA internal transcribed spacer (ITS) regions of S. fredii strains. Based on the sequences of the ITS regions, we divided the sequences of the S. fredii strains into two groups, type A and type B. A dot-matrix analysis indicated that the region flanked by tRNA-Ile and tRNA-Ala is longer in type A than in type B, whereas type B sequences possess longer regions upstream of tRNA-Ile and downstream of tRNA-Ala than those of the type A sequence. Restriction fragment length polymorphism of polymerase chain reaction product (PCR-RFLP) of the ITS region in the cloned plasmids as templates could reconstruct the PCR-RFLP pattern from the total DNA as a template. The results of Southern hybridization using the insert sequence between tRNA-Ile and tRNA-Ala in type A as a probe indicated differences in the copy numbers of the type A ITS regions among the strains tested. These results indicated that S. fredii strains possess the type A and type B sequences of the ITS regions at ratios of 3:0, 2:1, 1:2 or 0:3. These S. fredii strains may be useful biological materials for the study of intraspecific variations.
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UR - http://www.scopus.com/inward/citedby.url?scp=70349506846&partnerID=8YFLogxK
U2 - 10.1111/j.1747-0765.2009.00399.x
DO - 10.1111/j.1747-0765.2009.00399.x
M3 - Article
AN - SCOPUS:70349506846
VL - 55
SP - 627
EP - 633
JO - Soil Science and Plant Nutrition
JF - Soil Science and Plant Nutrition
SN - 0038-0768
IS - 5
ER -